对来自格鲁吉亚的 4 株耐多药鲍曼不动杆菌的比较基因组分析。
Comparative genomic analysis of four multidrug-resistant isolates of Acinetobacter baumannii from Georgia.
机构信息
Farlow Scientific Consulting, Lewiston, UT, USA; Department of Bacteriophage Therapeutics, Bacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
US Army Medical Research Directorate - Georgia (USAMRD-G), Tbilisi, Georgia.
出版信息
J Glob Antimicrob Resist. 2020 Jun;21:363-368. doi: 10.1016/j.jgar.2019.11.002. Epub 2019 Nov 12.
OBJECTIVES
This study reports the draft genomes of four newly isolated multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) isolates (0830, 0365, 4022, and 2846) from western Georgia to identify putative antimicrobial resistance genes (ARGs) and to determine the clonal subtypes of local clinical isolates.
METHODS
An Illumina MiSeq sequencer was used to perform whole-genome sequencing (WGS). The Vitek 2 automated system was used for microbial identification and antimicrobial resistance profiling.
RESULTS
Taxonomical identification as A. baumannii was confirmed by WGS. In silico analyses resolved their ARG content and clonal relatedness using the Oxford (Oxf) and Pasteur (Pas) multi-locus sequence typing schemes. Isolates 0365 and 4022 displayed similar allelic profiles corresponding to ST944Oxf/ST78Pas. Isolate 2846 displayed a different allelic profile consistent with ST19Pas/IC 1 (International or European Clone I) and exhibited a novel Oxford ST that was designated as 1868. Isolate 0830 displayed the ST78Pas allelic profile, similar to isolates 0365 and 4022, and also possessed a single allelic mismatch in the gpi gene, resulting in an ST1104Oxf allele profile in the Oxford typing scheme.
CONCLUSION
Circulating MDR A. baumannii exhibited genetic heterogeneity with variations in the structure and content of genomic A. baumannii resistance islands and encoded multiple putative ARGs. This report represents the first clonal subtype information and genomic characterization of MDR A. baumannii in Georgia and may inform future epidemiological investigations.
目的
本研究报告了从格鲁吉亚西部新分离的 4 株多药耐药（MDR）鲍曼不动杆菌（A.baumannii）（0830、0365、4022 和 2846）的基因组草图，以鉴定潜在的抗菌药物耐药基因（ARGs），并确定当地临床分离株的克隆亚型。
方法
使用 Illumina MiSeq 测序仪进行全基因组测序（WGS）。Vitek 2 自动化系统用于微生物鉴定和抗菌药物耐药谱分析。
结果
WGS 确认了分类学鉴定为鲍曼不动杆菌。通过牛津（Oxf）和巴斯德（Pas）多位点序列分型方案的基于计算机的分析，确定了它们的 ARG 含量和克隆相关性。分离株 0365 和 4022 显示出相似的等位基因谱，对应于 ST944Oxf/ST78Pas。分离株 2846 显示出不同的等位基因谱，与 ST19Pas/IC 1（国际或欧洲克隆 I）一致，并表现出一种新的牛津 ST，被命名为 1868。分离株 0830 显示出与 0365 和 4022 相似的 ST78Pas 等位基因谱，并且在 gpi 基因中存在单个等位基因不匹配，导致在牛津分型方案中具有 ST1104Oxf 等位基因谱。
结论
循环的 MDR 鲍曼不动杆菌表现出遗传异质性，其基因组鲍曼不动杆菌耐药岛的结构和内容存在差异，并编码多种潜在的 ARGs。本报告代表了格鲁吉亚首例 MDR 鲍曼不动杆菌的克隆亚型信息和基因组特征，可能为未来的流行病学调查提供信息。
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