Fukase Y, Fukase S, Sendo F
Department of Parasitology, Yamagata University School of Medicine.
Microbiol Immunol. 1988;32(6):621-33. doi: 10.1111/j.1348-0421.1988.tb01423.x.
We investigated the effect of supernatant from human spleen cell culture stimulated with a streptococcal preparation, OK-432 (OK sup), on the luminol-dependent chemiluminescence (CL) of human polymorphonuclear leukocytes (PMN). The fMLP-stimulated CL of PMN was markedly enhanced by the pretreatment with OK sup. This result indicates that OK sup contained the factor(s) that primes fMLP-stimulated CL of PMN. The priming factor(s) in OK sup was partially inactivated by the treatment at 56 C for 30 min and pH 2 or pH 10 treatments. Since the enhancing effect of OK sup on the CL was inhibited by the treatment of sodium azide and the addition of catalase or taurine, it was assumed that OK sup augments the activity of MPO-H2O2-HOCl system of fMLP-stimulated PMN.
我们研究了用链球菌制剂OK-432刺激人脾细胞培养上清液（OK sup）对人多形核白细胞（PMN）鲁米诺依赖性化学发光（CL）的影响。用OK sup预处理可显著增强PMN的fMLP刺激的CL。该结果表明OK sup中含有引发PMN的fMLP刺激的CL的因子。OK sup中的引发因子经56℃处理30分钟、pH 2或pH 10处理后部分失活。由于叠氮化钠处理以及添加过氧化氢酶或牛磺酸可抑制OK sup对CL的增强作用，因此推测OK sup增强了fMLP刺激的PMN的MPO-H2O2-HOCl系统的活性。
