Istituto di Genetica Molecolare "Luca Cavalli Sforza", Consiglio Nazionale delle Ricerche (CNR), 27100 Pavia, Italy.
Dipartimento di Biologia e Biotecnologie, Università di Pavia, 27100 Pavia, Italy.
Int J Mol Sci. 2019 Nov 15;20(22):5736. doi: 10.3390/ijms20225736.
The importance of determining at the cellular level the formation of DNA-protein complexes after radiation-induced lesions to DNA is outlined by the evidence that such interactions represent one of the first steps of the cellular response to DNA damage. These complexes are formed through recruitment at the sites of the lesion, of proteins deputed to signal the presence of DNA damage, and of DNA repair factors necessary to remove it. Investigating the formation of such complexes has provided, and will probably continue to, relevant information about molecular mechanisms and spatiotemporal dynamics of the processes that constitute the first barrier of cell defense against genome instability and related diseases. In this review, we will summarize and discuss the use of in situ procedures to detect the formation of DNA-protein complexes after radiation-induced DNA damage. This type of analysis provides important information on the spatial localization and temporal resolution of the formation of such complexes, at the single-cell level, allowing the study of heterogeneous cell populations.
在细胞水平上确定 DNA 损伤后 DNA-蛋白质复合物的形成对于阐明辐射诱导损伤后细胞对 DNA 损伤的反应的第一步之一，即这种相互作用具有重要意义。这些复合物是通过募集到损伤部位的、负责信号传递的蛋白质以及修复损伤所必需的 DNA 修复因子而形成的。研究这些复合物的形成提供了，并可能继续提供有关构成细胞抵御基因组不稳定性和相关疾病第一道防线的分子机制和时空动力学的相关信息。在这篇综述中，我们将总结和讨论使用原位程序来检测辐射诱导的 DNA 损伤后 DNA-蛋白质复合物的形成。这种分析类型提供了关于此类复合物形成的空间定位和时间分辨率的重要信息，在单细胞水平上，可以研究异质细胞群体。
