University of South Alabama Mitchell Cancer Institute, 1660 Springhill Ave, Mobile, AL 36607, USA.
University of South Alabama Mitchell Cancer Institute, 1660 Springhill Ave, Mobile, AL 36607, USA.
Curr Opin Biotechnol. 2019 Feb;55:30-35. doi: 10.1016/j.copbio.2018.08.001. Epub 2018 Aug 13.
Detection and characterization of DNA damage is essential for evaluating genotoxicity, monitoring DNA repair, developing biomarkers for exposures, and evaluating the efficacy of chemotherapies. These diverse applications for DNA damage measurements have spurred the continual development and refinement of methodologies for detecting, characterizing, and quantifying DNA damage from isolated DNA and in cells and tissues. Current damage detection methods cover a wide range of techniques from radiolabeling to mass spectrometry, and use of these techniques varies widely based on expense, expertise, and knowledge of adduct formation. More generalizable, easy-to-use methods for detecting and quantifying DNA damage are needed, and there has been an emergence of fluorescence-based methodologies to address this need. Developments in these fluorescence-based strategies are reviewed here.
检测和描述 DNA 损伤对于评估遗传毒性、监测 DNA 修复、开发暴露生物标志物以及评估化疗效果至关重要。这些对 DNA 损伤测量的不同应用推动了不断发展和完善的方法学的发展，以检测、描述和量化来自分离的 DNA 以及细胞和组织中的 DNA 损伤。目前的损伤检测方法涵盖了从放射性标记到质谱分析的广泛技术，这些技术的使用因费用、专业知识和加合物形成的知识而异。需要更具普遍性、更易于使用的方法来检测和量化 DNA 损伤，并且已经出现了基于荧光的方法来满足这一需求。这里综述了这些基于荧光的策略的发展。
