Shugart Holly, Ebert Timothy, Gmitter Frederick, Rogers Michael
Department of Plant Pathology, Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Department of Entomology and Nematology, Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Insects. 2019 Nov 15;10(11):407. doi: 10.3390/insects10110407.
The invasive Asian citrus psyllid, (Hemiptera: Liviidae), is the primary vector of the phloem-infecting bacterium, Liberibacter asiaticus. L. asiaticus is the putative causal agent of Huanglongbing (HLB) disease, a destructive disease of . While many species are susceptible to probing and HLB disease, there are marked behavioral differences in probing responses and Ca. Liberibacter asiaticus infection severity among species. Using four mandarin hybrid selections and pummelo plants variably resistant to probing, oviposition, and survival, we explored probing differences using electropenetrography (EPG), conducted an oviposition and survival study, and determined host plant metabolites using gas-chromatography mass-spectroscopy (GC-MS). We found thirty-seven probing variables to be significantly different among tested mandarin selections and pummelo, in addition to differential oviposition and survivorship abilities on tested plants. We found sixty-three leaf metabolites with eight being significantly different among tested mandarin selections and pummelo. Detailed analysis of probing behavior, oviposition, survivorship, and host plant metabolite concentrations reveals the complex, layered resistance mechanisms utilized by resistant against probing. EPG is a powerful technology for screening Asian citrus psyllid resistant to elucidate host plant-vector interactions, with an aim to minimize vector probing and eliminate the spread of the bacterial pathogen, Ca. L. asiaticus.
入侵性亚洲柑橘木虱（半翅目：木虱科）是韧皮部感染细菌——亚洲韧皮杆菌的主要传播媒介。亚洲韧皮杆菌被认为是黄龙病（HLB）的致病因子，黄龙病是柑橘的一种毁灭性病害。虽然许多柑橘品种易受亚洲柑橘木虱的刺探和黄龙病影响，但不同柑橘品种在刺探反应和亚洲韧皮杆菌感染严重程度方面存在显著的行为差异。我们使用了四种对亚洲柑橘木虱刺探、产卵和存活具有不同抗性的柑橘杂交品种和柚类植株，通过直流刺探电位图谱技术（EPG）探究刺探差异，进行了产卵和存活研究，并使用气相色谱 - 质谱联用仪（GC - MS）测定宿主植物代谢物。我们发现，除了在受试植株上产卵和存活能力存在差异外，在受试柑橘品种和柚类之间，有37个亚洲柑橘木虱刺探变量存在显著差异。我们还发现了63种叶片代谢物，其中8种在受试柑橘品种和柚类之间存在显著差异。对刺探行为、产卵、存活和宿主植物代谢物浓度的详细分析揭示了抗性柑橘品种对亚洲柑橘木虱刺探所利用的复杂、多层次的抗性机制。EPG是一种强大的技术，可用于筛选抗亚洲柑橘木虱的柑橘品种，以阐明宿主植物与媒介之间的相互作用，旨在尽量减少媒介刺探并消除细菌性病原体——亚洲韧皮杆菌的传播。
