College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; College of Biological Science and Technology, Hubei Minzu University, Enshi 445000, China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Ningbo Research Institute, Zhejiang University, Ningbo 315100, China.
Int J Biol Macromol. 2020 Jul 1;154:1478-1489. doi: 10.1016/j.ijbiomac.2019.11.029. Epub 2019 Nov 14.
In this study, the gelatin/zein nanofibrous membranes with incorporation of nano-hydroxyapatite (nHA) were fabricated via electrospinning. The gelatin/zein/nHA solutions as the spinning dispersions showed the viscoelastic property with a shear-thinning non-Newtown behavior. The incorporated nHA particles in the composite nanofibrous membranes did not affect the average fiber diameter significantly, but induced agglomeration and nodules at higher contents. It was found that nHA was dispersed within the gelatin/zein nanofibers by the formed hydrogen bonding, resulting in a more extensional structure of the proteins. The addition of nHA significantly increased the hydrophobicity of the nanofibrous membranes, due to the lower ratio of polar groups exposed outside. However, the incorporation of nHA improved the mechanical property at a low content of 10%, but resulted in a more brittle and fragile property at higher contents. The copper adsorption capacity of the gelatin/zein nanofibrous membrane increased from 27.2 to 67.8 mg/g after the addition of up to 50% nHA. This work suggests the potentials of the electrospun gelatin/zein/nHA nanofibrous membranes as desirable materials for metal removal applications.
在这项研究中，通过静电纺丝制备了掺入纳米羟基磷灰石（nHA）的明胶/玉米醇溶蛋白纳米纤维膜。作为纺丝分散体的明胶/玉米醇溶蛋白/nHA 溶液具有剪切变稀的非牛顿行为的粘弹性。在复合纳米纤维膜中掺入的 nHA 颗粒对平均纤维直径没有显著影响，但在较高含量下会引起团聚和结节。研究发现，nHA 通过形成氢键分散在明胶/玉米醇溶蛋白纳米纤维中，从而使蛋白质具有更伸展的结构。由于暴露在外的极性基团比例较低，nHA 的添加显著增加了纳米纤维膜的疏水性。然而，在添加低含量（10%）的 nHA 时，nHA 的掺入提高了机械性能，但在较高含量时，会导致更脆的性质。明胶/玉米醇溶蛋白纳米纤维膜的铜吸附容量从 27.2 增加到 67.8 mg/g，在添加高达 50%的 nHA 后。这项工作表明，静电纺丝的明胶/玉米醇溶蛋白/nHA 纳米纤维膜具有作为去除金属应用的理想材料的潜力。
