Bkaily G, Peyrow M, Yamamoto T, Sculptoreanu A, Jacques D, Sperelakis N
Department of Physiology and Biophysics, Faculty of Medicine, University of Sherbrooke (CHUS), Québec, Canada.
Mol Cell Biochem. 1988 Mar-Apr;80(1-2):59-72. doi: 10.1007/BF00231004.
The whole-cell voltage clamp technique was used to study the slow inward currents and K+ outward currents in single heart cells of embryonic chick and in rabbit aortic cells. In single heart cells of 3-day-old chick embryo three types of slow inward Na+ currents were found. The kinetics and the pharmacology of the slow INa were different from those of the slow ICa in older embryos. Two types of slow inward currents were found in aortic single cells of rabbit; angiotensin II increased the sustained type and d-cAMP and d-cGMP decreased the slow transient component. Two types of outward K+ currents were found in both aortic and heart cells. Single channel analysis demonstrated the presence of a high single K+ channel conductance in aortic cells. In cardiac and vascular smooth muscles, slow inward currents do share some pharmacological properties, although the regulation of these channels by cyclic nucleotides and several drugs seems to be different.
采用全细胞膜片钳技术研究了鸡胚单心肌细胞和兔主动脉细胞中的缓慢内向电流和钾离子外向电流。在3日龄鸡胚的单心肌细胞中发现了三种类型的缓慢内向钠离子电流。缓慢内向钠电流的动力学和药理学特性与 older embryos中缓慢内向钙电流的不同。在兔主动脉单细胞中发现了两种类型的缓慢内向电流；血管紧张素II增加了持续性类型，而二丁酰环磷腺苷（d-cAMP）和二丁酰环磷鸟苷（d-cGMP）降低了缓慢瞬态成分。在主动脉细胞和心肌细胞中均发现了两种类型的外向钾电流。单通道分析表明主动脉细胞中存在高单钾通道电导。在心脏和血管平滑肌中，缓慢内向电流确实具有一些药理学特性，尽管环核苷酸和几种药物对这些通道的调节似乎有所不同。
