Snyder R D, Davis G F
Merrell Dow Research Institute, Cincinnati, OH 45215.
Mutat Res. 1988 Sep-Oct;209(1-2):51-6. doi: 10.1016/0165-7992(88)90110-8.
Deoxynucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools were analyzed in 4 mammalian cell lines following treatment with UV-C (254 nm), UV-A (365 nm) or the carcinogen, 1-methyl-3-nitro-1-nitrosoguanidine (MNNG). No substantial alterations in dNTP pool levels were observed in any treatment group. However, the cellular conversions of exogenously added deoxycytidine and deoxyguanosine to the corresponding triphosphates were inhibited 30-97% by UV-C and MNNG treatment. In addition, the conversion of dGuo to GTP and deoxyadenosine to ATP were inhibited 25-50% in CHO cells by mutagen treatment. The data do not support the notion that modulation of specific dNTP pools is a general feature of mutagen treatment in mammalian cells, but so suggest a mutagen-sensitivity of deoxynucleoside metabolism.
在用紫外线C（254纳米）、紫外线A（365纳米）或致癌物1-甲基-3-硝基-1-亚硝基胍（MNNG）处理后，对4种哺乳动物细胞系中的脱氧核苷三磷酸（dNTP）和核糖核苷三磷酸（rNTP）库进行了分析。在任何处理组中均未观察到dNTP库水平有实质性改变。然而，紫外线C和MNNG处理可使外源添加的脱氧胞苷和脱氧鸟苷向相应三磷酸酯的细胞转化率降低30 - 97%。此外，诱变处理可使CHO细胞中dGuo向GTP以及脱氧腺苷向ATP的转化率降低25 - 50%。这些数据并不支持特定dNTP库的调节是哺乳动物细胞诱变处理的普遍特征这一观点，但表明脱氧核苷代谢具有诱变敏感性。
