诱变处理未影响的小鼠FM3A细胞系的脱氧核苷三磷酸库。
Deoxynucleoside triphosphate pool of mouse FM3A cell lines unaffected by mutagen treatment.
作者信息
Hyodo M, Ito N, Suzuki K
出版信息
Biochem Biophys Res Commun. 1984 Aug 16;122(3):1160-5. doi: 10.1016/0006-291x(84)91213-0.
The size of deoxynucleoside triphosphate pool in cultured mouse FM3A cells and mutator mutants isolated from this cell line was determined by high-pressure liquid chromatography after treatment of the cells with ultraviolet light, N-methyl-N'-nitro-N-nitrosoguanidine or mitomycin C. The results showed that, in all the FM3A cell clones, no large increase in the dATP or TTP pool was induced after treatment, while in some cases 40-50% decrease in dCTP pool was observed. It is concluded that the induction of large increase in dNTP pool is not the general effect of the mutagens.
用紫外线、N-甲基-N'-硝基-N-亚硝基胍或丝裂霉素C处理培养的小鼠FM3A细胞及其从该细胞系分离出的诱变突变体后，通过高压液相色谱法测定了脱氧核苷三磷酸池的大小。结果表明，在所有FM3A细胞克隆中，处理后dATP或TTP池均未大幅增加，而在某些情况下，观察到dCTP池减少了40-50%。得出的结论是，诱变剂普遍不会导致dNTP池大幅增加。
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