Funing People's Hospital, Yancheng, Jiangsu Province 224000, China.
Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province, China.
J Photochem Photobiol B. 2020 Jan;202:111698. doi: 10.1016/j.jphotobiol.2019.111698. Epub 2019 Nov 9.
Gastric cancer was a foremost one among the majority of regular carcinoma cases globally. Even the achievements on enhanced treatment approaches and early findings cannot decrease the mortality and morbidity ranges of gastric cancer. This current work was planned to develop Morus nigra-loaded zinc oxide nanoparticles (MN-ZnONPs) and to evaluate the different characteristic methods likes UV-vis spectroscopy, TEM, SEM, FT-IR, EDX and XRD. Furthermore, the anticancer effect of MN-ZnONPs against AGS cells were analysed via cell viability, apoptotic morphological variations by AO/EtBr, alterations of mitochondrial membrane potential (MMP), cell cycle arrest, lipid peroxidation status (TBARS), antioxidants (SOD, GSH and CAT) and generation of ROS. Moreover, the status of apoptosis gene such as Bax, caspase-9, caspase-3 and Bcl-2 expressions was analysed by using RT-PCR techniques. We observed the synthesized MN-ZnONPs have a spherical shape, crystalline nature and present different functional groups. We also observed that gastric cancer cells demonstrated in cell death by MN-ZnONPs treatments. The MN-ZnONPs induced apoptosis by enhanced formation of ROS, decreased MMP, apoptotic morphological modifications were evaluated by AO/EtBr, increased lipid peroxidation, decreased antioxidants and induced cell cycle arrest were observed. Furthermore, to confirm the molecular mechanism demonstrated of MN-ZnONPs to induce apoptosis by altering the gene expressions of apoptosis markers were observed.
胃癌是全球大多数常见癌症病例中的首要癌症之一。即使在治疗方法的改进和早期发现方面取得了成就，也不能降低胃癌的死亡率和发病率范围。本研究旨在制备桑椹负载氧化锌纳米粒子（MN-ZnONPs），并通过紫外可见光谱、TEM、SEM、FT-IR、EDX 和 XRD 等不同特征方法进行评价。此外，通过细胞活力、AO/EtBr 诱导的凋亡形态变化、线粒体膜电位（MMP）改变、细胞周期阻滞、脂质过氧化状态（TBARS）、抗氧化剂（SOD、GSH 和 CAT）和 ROS 生成来分析 MN-ZnONPs 对 AGS 细胞的抗癌作用。此外，还通过 RT-PCR 技术分析了凋亡基因如 Bax、caspase-9、caspase-3 和 Bcl-2 的表达状态。我们观察到合成的 MN-ZnONPs 具有球形形状、结晶性质和存在不同的官能团。我们还观察到 MN-ZnONPs 处理后胃癌细胞表现出细胞死亡。MN-ZnONPs 通过增强 ROS 的形成、降低 MMP、通过 AO/EtBr 评估凋亡形态变化、增加脂质过氧化、降低抗氧化剂和诱导细胞周期阻滞来诱导细胞凋亡。此外，为了证实 MN-ZnONPs 通过改变凋亡标志物的基因表达诱导细胞凋亡的分子机制，观察到了这一机制。
