Instituto de Investigación para La Gestión Integrada de Zonas Costeras, Campus de Gandía, Universitat Politècnica de València, C/ Paranimf 1, 46730, Grao de Gandía, Valencia, Spain.
Instituto de Investigación para La Gestión Integrada de Zonas Costeras, Campus de Gandía, Universitat Politècnica de València, C/ Paranimf 1, 46730, Grao de Gandía, Valencia, Spain.
Anal Chim Acta. 2020 Jan 6;1093:160-167. doi: 10.1016/j.aca.2019.09.055. Epub 2019 Sep 24.
In this study, poly(butyl methacrylate-co-ethyleneglycol dimethacrylate) polymeric monoliths were in situ developed within 0.75 mm i.d. poly(ethylene-co-tetrafluoroethylene) (ETFE) tubing by UV polymerization via three different free-radical initiators (α,α'-azobisisobutyronitrile (AIBN), 2,2-dimethoxy-2-phenylacetophenone (DMPA) and 2-methyl-4'-(methylthio)-2-morpholinopropiophenone (MTMPP). The influence of the nature of each photo-initiator and irradiation time on the morphological features of the polymer was investigated by scanning electron microscopy, and the chromatographic properties of the resulting microbore columns were evaluated using alkyl benzenes as test substances. The beds photo-initiated with MTMPP gave the best performance (minimum plate heights of 38 μm for alkyl benzenes) and exhibited a satisfactory reproducibility in the chromatographic parameters (RSD < 11%). These monolithic columns were also successfully applied to the separation of phenylurea herbicides, proteins and a tryptic digest of β-casein.
在这项研究中，通过紫外光聚合，在 0.75mm ID 的聚（乙烯-共-四氟乙烯）（ETFE）管内原位合成了聚（甲基丙烯酸丁酯-共-乙二醇二甲基丙烯酸酯）聚合体整体柱，使用了三种不同的自由基引发剂（α,α'-偶氮二异丁腈（AIBN）、2,2-二甲氧基-2-苯乙酮（DMPA）和 2-甲基-4'-(甲硫基)-2-吗啉基丙基苯酮（MTMPP）。通过扫描电子显微镜研究了每种光引发剂的性质和辐照时间对聚合物形态特征的影响，并使用烷基苯作为测试物质评估了所得微柱的色谱性能。用 MTMPP 引发的床层性能最佳（烷基苯的最小板高为 38μm），且在色谱参数的重现性良好（RSD<11%）。这些整体柱还成功地应用于苯脲类除草剂、蛋白质和β-酪蛋白的胰蛋白酶消化物的分离。
