Instituto de Investigación para la Gestión Integrada de Zonas Costeras, Campus de Gandía, Universitat Politècnica de València, C/ Paranimf 1, 46730 Grao de Gandía, Valencia, Spain.
Instituto de Investigación para la Gestión Integrada de Zonas Costeras, Campus de Gandía, Universitat Politècnica de València, C/ Paranimf 1, 46730 Grao de Gandía, Valencia, Spain.
Talanta. 2018 Sep 1;187:216-222. doi: 10.1016/j.talanta.2018.05.026. Epub 2018 May 16.
In this study, porous polymer monoliths were in situ synthesized in fluoropolymers tubing to prepare microbore HPLC columns. To ensure the formation of robust homogeneous polymer monoliths in these housing supports, the inner surface of fluoropolymer tubing was modified in a two-step photografting process. Raman spectroscopy and scanning electron microscopy (SEM) confirmed the successful modification of the inner poly(ethylene-co-tetrafluoroethylene) (ETFE) wall and the subsequent attachment of a monolith onto the wall. Poly(glycidyl methacrylate-co-divinylbenzene), poly(butyl methacrylate-co-ethyleneglycol dimethacrylate) and poly(styrene-co-divinylbenzene) monoliths were in situ synthesized by thermal polymerization within the confines of surface vinylized ETFE tubes. The resulting monoliths exhibited good permeability and mechanical stability (pressure resistance up to 9 MPa). The chromatographic performance of these different monolithic columns was evaluated via the separation of alkyl benzenes and proteins in a conventional HPLC system.
在这项研究中，通过在氟聚合物管中就地合成多孔聚合物整体材料来制备微径高效液相色谱柱。为了确保在这些外壳支撑物中形成坚固的均一聚合物整体材料，采用两步光接枝法对氟聚合物管的内表面进行了改性。拉曼光谱和扫描电子显微镜（SEM）证实了聚（乙烯-共-四氟乙烯）（ETFE）壁的成功改性以及随后在壁上附着的整体材料。通过在表面乙烯化 ETFE 管的限制范围内进行热聚合，原位合成了聚（甲基丙烯酸缩水甘油酯-共-二乙烯基苯）、聚（甲基丙烯酸丁酯-共-乙二醇二甲基丙烯酸酯）和聚（苯乙烯-共-二乙烯基苯）整体材料。得到的整体材料表现出良好的渗透性和机械稳定性（耐压高达 9 MPa）。通过在常规高效液相色谱系统中分离烷基苯和蛋白质，评估了这些不同整体材料柱的色谱性能。
