Wang Chun Ming, Sun Ying Li, Lin Chang Jun, Wang Ming Yu, Niu Yue, Li Xiao Feng, Feng Hu Yuan
School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Yi Chuan. 2019 Nov 20;41(11):1067-1072. doi: 10.16288/j.yczz.19-181.
Genetic analysis is an important part of undergraduate genetics teaching and tetrad analysis is unique and integral for genetic analysis of fungi. The ordered tetrad in Neurospora is an important material for genetic analysis, which can not only be used to study recombination between genes and centromeres, but also between genes themselves, as well as study the fine cross patterns between non-sister chromatids of homologous chromosomes. However, in textbooks and related professional journals, there is a lack of specific introduction to the induction methods of the seven basic class asci used in two genes analysis. In the present paper, we designed a table presenting the correlation between the three tetrad types (PD, NPD, T) and the four segregation pattern groups (Ⅰ Ⅰ, Ⅱ Ⅱ, Ⅰ Ⅱ, Ⅱ Ⅰ) to visually show the 12 possible combinations (3×4=12). Then five of them were excluded through the "×" symbol and in addition with three comments attached with the table, thus finally we obtained seven basic ascus types. We hope that this analytical method can assist the teaching of ordered tetrad analysis in Neurospora.
遗传分析是本科遗传学教学的重要组成部分，而四分体分析对于真菌的遗传分析具有独特性和不可或缺性。粗糙脉孢菌的顺序四分体是遗传分析的重要材料，它不仅可用于研究基因与着丝粒之间的重组，还可用于研究基因自身之间的重组，以及研究同源染色体非姐妹染色单体之间的精细交叉模式。然而，在教科书和相关专业期刊中，缺乏对两基因分析中使用的七种基本类型子囊诱导方法的具体介绍。在本文中，我们设计了一个表格，展示了三种四分体类型（PD、NPD、T）与四种分离模式组（ⅠⅠ、ⅡⅡ、ⅠⅡ、ⅡⅠ）之间的相关性，以直观呈现12种可能的组合（3×4 = 12）。然后通过“×”符号排除其中5种，并在表格中附上三条注释，最终得到七种基本子囊类型。我们希望这种分析方法能够辅助粗糙脉孢菌顺序四分体分析的教学。
