Russell P J, Petersen R C, Wagner S
Biology Department, Reed College, Portland, OR 97202.
Mol Gen Genet. 1988 Mar;211(3):541-4. doi: 10.1007/BF00425714.
The genetic segregation of ribosomal DNA (rDNA) in Neurospora crassa was analyzed by exploiting restriction fragment length polymorphisms in the nontranscribed spacer (NTS) sequences of nine laboratory wild-type strains and wild-collected strains. In an analysis of random spore progeny from seven crosses, and of ordered tetrads from two of those crosses the rDNA was shown to be inherited in a simple, stable Mendelian fashion, exhibiting an approximately 1:1 ratio of the two parental rDNA types. No meiotic recombinants were detected among the progeny, indicating that non-sister-chromatid crossing over is highly suppressed in the rDNA region. The basis for this suppression of meiotic recombination is not known.
通过利用9个实验室野生型菌株和野生采集菌株非转录间隔区（NTS）序列中的限制性片段长度多态性，分析了粗糙脉孢菌核糖体DNA（rDNA）的遗传分离情况。在对7个杂交组合的随机孢子后代以及其中2个杂交组合的顺序四分子进行分析时，rDNA显示以简单、稳定的孟德尔方式遗传，两种亲本rDNA类型的比例约为1:1。在后代中未检测到减数分裂重组体，这表明rDNA区域中姐妹染色单体间的交叉互换受到高度抑制。这种减数分裂重组抑制的基础尚不清楚。
