Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou 434025, China; Institute of Entomology, College of Agriculture, Yangtze University, Jingzhou 434025, China.
Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou 434025, China; Institute of Entomology, College of Agriculture, Yangtze University, Jingzhou 434025, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Jun;38:100818. doi: 10.1016/j.cbd.2021.100818. Epub 2021 Feb 19.
The Chinese citrus fruit fly, Bactrocera minax (Enderlein), a devastating pest in citrus, has an obligatory diapause at the pupal stage. Although the physiology of obligatory diapause has been well described, the molecular mechanisms underlying the process remain unknow. Here we investigated the molecular mechanisms of obligatory diapause induction in B. minax using high-throughput RNA-Seq data from second-instar larva (2L), third-instar larva (3L) and pupa (P) stages. A total of 116,402 unigenes were obtained, of which 54,781 unigenes were successfully annotated in public databases, and the differentially expressed genes in the 3L vs 2L, P vs 2L, and P vs 3L comparisons were identified. The cluster co-expression patterns of the differentially expressed genes revealed that significantly differentially-expressed genes in the pupal stage were predicted to be related to diapause induction. All differentially expressed genes were investigated by GO functional and KEGG pathway analysis, and the results showed that genes involved in processes such as 20-hydroxyecdysone (20E) biosynthesis, cell cycle and metabolic pathways are likely related to obligatory diapause induction in B. minax. These results provide important information on the transcriptome of the Chinese citrus fruit fly that can be used for further functional studies as well as contributing to our understanding of the molecular basis of obligatory diapause induction and suggesting potential molecular targets for the control of this pest.
中国桔小实蝇（Bactrocera minax （Enderlein））是一种严重危害柑橘的毁灭性害虫，其在蛹期存在强制性滞育。尽管强制性滞育的生理学已经得到很好的描述，但该过程的分子机制仍不清楚。在这里，我们使用来自二龄幼虫（2L）、三龄幼虫（3L）和蛹（P）阶段的高通量 RNA-Seq 数据研究了 B. minax 中强制性滞育诱导的分子机制。共获得了 116402 个 unigenes，其中 54781 个 unigenes成功注释到公共数据库中，并且鉴定了 3L 与 2L、P 与 2L 和 P 与 3L 比较中的差异表达基因。差异表达基因的聚类共表达模式表明，蛹期显著差异表达的基因预测与滞育诱导有关。通过 GO 功能和 KEGG 途径分析所有差异表达基因，结果表明，参与 20-羟蜕皮酮（20E）生物合成、细胞周期和代谢途径等过程的基因可能与 B. minax 中的强制性滞育诱导有关。这些结果为中国桔小实蝇的转录组提供了重要信息，可用于进一步的功能研究，并有助于我们理解强制性滞育诱导的分子基础，并为控制这种害虫提供潜在的分子靶标。
