Tode Jan, Richert Elisabeth, Koinzer Stefan, Klettner Alexa, von der Burchard Claus, Brinkmann Ralf, Lucius Ralph, Roider Johann
Christian-Albrechts-University of Kiel, University Medical Center, Department of Ophthalmology, Arnold-Heller-Str. 3, Kiel, Germany.
Institute for Biomedical Optics, University of Lübeck and Medical Laser Center Lübeck GmbH, Peter-Monnik-Weg 4, Lübeck, Germany.
Transl Vis Sci Technol. 2019 Nov 13;8(6):11. doi: 10.1167/tvst.8.6.11. eCollection 2019 Nov.
To investigate the effect of selective retina therapy (SRT) on age-related macular degeneration (AMD)-like alterations of retinal pigment epithelium (RPE) and Bruch's membrane (BrM) in AMD mouse models as therapeutic approach for the treatment of dry AMD.
In B6.129P2- /J (ApoE) and B6.129X1-Nfe2I2 /J (NRF2), one randomized eye of each mouse in groups of 15 mice was treated by SRT (532 nm, 300 ms, ∼1.4-μs pulse, 100 Hz, 50-μm spot), the fellow eye and healthy C57BL/6J mice served as controls. Clinical examinations were obtained at treatment day and 1 month later, followed by enucleation to analyze BrM thickness and ultrastructural RPE morphology.
Nearly all ApoE and NRF2 mice showed AMD-like retinal alterations. BrM thickness was increased in both mouse models, RPE had vacuoles within the cell body and shortened apical microvilli. SRT neither affected neuroretinal anatomy nor function. BrM thickness as well as AMD-like ultrastructural alterations of the RPE were significantly reduced in laser-treated eyes compared with fellow control and untreated control eyes.
SRT reduces BrM thickness and AMD-like RPE alterations in AMD mouse models without damage to structural or functional properties of neuroretina. It may be a prophylactic or therapeutic option for dry AMD.
SRT shows therapeutic effectivity in murine AMD models and might therefore become an option for the treatment of dry AMD.
研究选择性视网膜治疗（SRT）对年龄相关性黄斑变性（AMD）小鼠模型中视网膜色素上皮（RPE）和布鲁赫膜（BrM）的AMD样改变的影响，作为干性AMD的治疗方法。
在B6.129P2 - /J（ApoE）和B6.129X1 - Nfe2I2 /J（NRF2）小鼠中，将每组15只小鼠的一只随机眼睛接受SRT治疗（532 nm，300 ms，~1.4 μs脉冲，100 Hz，50 μm光斑），对侧眼和健康的C57BL/6J小鼠作为对照。在治疗当天和1个月后进行临床检查，随后摘除眼球以分析BrM厚度和RPE超微结构形态。
几乎所有ApoE和NRF2小鼠都表现出AMD样视网膜改变。两种小鼠模型中BrM厚度均增加，RPE细胞体内有液泡，顶端微绒毛缩短。SRT既不影响神经视网膜的解剖结构也不影响其功能。与对侧对照眼和未治疗的对照眼相比，激光治疗眼的BrM厚度以及RPE的AMD样超微结构改变明显减少。
SRT可减少AMD小鼠模型中的BrM厚度和AMD样RPE改变，而不损害神经视网膜的结构或功能特性。它可能是干性AMD的一种预防或治疗选择。
SRT在小鼠AMD模型中显示出治疗效果，因此可能成为干性AMD治疗的一种选择。
