对二甲苯调节小鼠巨噬细胞氧化应激和炎症：在动脉粥样硬化中的潜在意义。
p-Cymene Modulate Oxidative Stress and Inflammation in Murine Macrophages: Potential Implication in Atherosclerosis.
机构信息
Department of Clinical Laboratory and Nutritional Sciences, University of Massachusetts, Lowell, MA, United States.
出版信息
Cardiovasc Hematol Agents Med Chem. 2020;18(2):151-157. doi: 10.2174/1871525717666191118112310.
BACKGROUND
p-Cymene (p-CYM) is a common chemical used in air fresheners.
OBJECTIVE
The study was designed to investigate the molecular effect of p-CYM on macrophages.
MATERIALS AND METHODS
Macrophages (RAW 264.7) were treated with p-CYM (50 uM/L, 150 uM/L and 250 uM/L) for 6 hours, and 24 hours). Gene involved in inflammation, such as the Tumor Necrosis Factor-alpha (TNF-α), and the Monocyte Chemoattractant Protein-1 (MCP-1) and other genes known for their antioxidant activity such as the Paraoxonase 1 (PON-1) were analyzed.
RESULTS
Cells treated with p-CYM have shown 30% up-regulation of MCP-1 after 24 hour of exposure; and also a differential up-regulation of TNF-α. However, treatment with p-CYM has resulted in a considerable (37%) dose-dependent downregulation of PON-1 after 24 hours of exposure. PON-1 is known for its antioxidant properties protecting High-Density Lipoproteins (HDL) from oxidation.
CONCLUSION
Our findings demonstrate that exposure to p-CYM over time promotes oxidative stress by downregulating antioxidants genes as shown in PON-1 and also stimulates inflammation, a key process during the initiation and progression of atherosclerosis.
背景
对伞花烃（p-CYM）是空气清新剂中常用的一种化学物质。
目的
本研究旨在研究 p-CYM 对巨噬细胞的分子作用。
材料与方法
用 p-CYM（50μM/L、150μM/L 和 250μM/L）处理巨噬细胞（RAW 264.7）6 小时和 24 小时。分析了炎症相关基因，如肿瘤坏死因子-α（TNF-α）和单核细胞趋化蛋白-1（MCP-1），以及其他具有抗氧化活性的基因，如对氧磷酶 1（PON-1）。
结果
暴露于 p-CYM 的细胞在 24 小时后显示 MCP-1 上调 30%；TNF-α也呈现差异上调。然而，p-CYM 的处理导致 PON-1 在 24 小时的暴露后呈现出明显的（37%）剂量依赖性下调。PON-1 具有抗氧化特性，可保护高密度脂蛋白（HDL）免受氧化。
结论
我们的研究结果表明，随着时间的推移，暴露于 p-CYM 会通过下调 PON-1 等抗氧化基因来促进氧化应激，同时还会刺激炎症，这是动脉粥样硬化起始和进展过程中的一个关键过程。
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