Sibley School of Mechanical and Aerospace Engineering , Cornell University , Ithaca , New York 14853 , United States.
Division of Nutritional Sciences , Cornell University , Ithaca , New York 14853 , United States.
Anal Chem. 2019 Dec 3;91(23):14824-14828. doi: 10.1021/acs.analchem.9b04180. Epub 2019 Nov 20.
The centrifuge is the gold standard for lab-based sample processing. While extremely efficient and robust, centrifuges are seldom used in the field due to the high-power requirements, size, and operational complexity. The lack of viable alternatives for remote sample collection has crippled the ability for mobile practitioners in human and animal medicine to reliably collect blood samples from their patients. There is no truly resource-independent solution that is able to perform highly efficient blood-plasma separation. Here, we describe our initial efforts in developing the High Efficiency Rapid Magnetic Erythrocyte Separator (H.E.R.M.E.S) sleeve, an apparatus that uses a magnetic bead-based separation assay in a scaled-up form factor to achieve highly efficient separation of erythrocytes from plasma within a short amount of time. The sleeve is easy-to-use, is completely resource independent, and achieves highly efficient separation in sample volumes as large as 1 mL by means of a unique mixing scheme. We demonstrate the performance of the sleeve with human blood samples and compare it against conventional end-over-end mixing.
离心机是实验室样本处理的金标准。尽管离心机非常高效且强大，但由于其高功率要求、体积大和操作复杂性，很少在现场使用。由于缺乏可行的远程样本采集替代方案，人类和动物医学中的移动从业者无法可靠地从患者身上采集血液样本。目前还没有真正独立于资源的解决方案能够实现高效的血液-血浆分离。在这里，我们描述了我们在开发高效快速磁红细胞分离器（H.E.R.M.E.S）套管方面的初步努力，该装置采用基于磁性珠的分离测定法，在放大的外形尺寸下实现红细胞在短时间内从血浆中高效分离。该套管易于使用，完全独立于资源，并且通过独特的混合方案，在高达 1 毫升的样本量下实现高效分离。我们用人血样本验证了套管的性能，并将其与传统的颠倒混合进行了比较。
