Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China; Engineering Research Center for Application Technology of Green Agricultural Microbes of Yunnan College, Kunming, Yunnan 650500, China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, China; Engineering Research Center for Application Technology of Green Agricultural Microbes of Yunnan College, Kunming, Yunnan 650500, China.
Microbiol Res. 2020 Jan;231:126370. doi: 10.1016/j.micres.2019.126370. Epub 2019 Nov 7.
The role of the gut microbiome in animal health has become increasingly evident. Although the structure of the gut microbiome of A. mellifera is well known, little is known about the dynamic change across different developmental stages. In this study, we explored the dynamic changes of the gut microbiota of A. mellifera at different developmental stages covering the whole life cycle using high-throughput 16S rRNA gene sequencing. The results indicated that the core (shared) gut microbiota changes significantly among different developmental stages. The diversity of the bacterial community in workers among different ages was significantly different. In addition, by comparing the core gut microbiota among different-aged workers, we found that newly emerged workers had fewer core microbiota. Three genera, Gilliamella, Frischella, and Snodgrassella, were significantly colonized at 1 day poste mergence (dpe); Lactobacillus, Bifidobacterium, Commensalibacter were significantly colonized at 3 dpe and significantly reduced with Gilliamella. Lactobacillus kunkeei and Bartonella were significantly colonized at 12 dpe and were significantly decreased with Lactobacillus helsingborgensis. Commensalibacter and Bifidobacterium were significantly decreased at 25 dpe, and Bacteroides, Escherichia-Shigella, and Porphyromonadaceae were significantly decreased between 19 and 25 dpe. Our results reveal the succession of the gut microbiota of workers from birth to senescence, which provides a theoretical basis for further exploring the roles of gut microbiota during different developmental stages.
肠道微生物组在动物健康中的作用已变得越来越明显。尽管蜜蜂的肠道微生物组结构已经众所周知，但对于不同发育阶段的动态变化却知之甚少。在这项研究中，我们使用高通量 16S rRNA 基因测序技术，研究了蜜蜂在整个生命周期中不同发育阶段肠道微生物组的动态变化。结果表明，核心（共享）肠道微生物组在不同发育阶段之间发生了显著变化。不同年龄工蜂的细菌群落多样性存在显著差异。此外，通过比较不同年龄工蜂的核心肠道微生物组，我们发现新出现的工蜂的核心微生物组较少。三个属，Gilliamella、Frischella 和 Snodgrassella，在刚出房后 1 天（1 dpe）大量定植；Lactobacillus、Bifidobacterium 和 Commensalibacter 在 3 dpe 大量定植，并与 Gilliamella 一起显著减少。Lactobacillus kunkeei 和 Bartonella 在 12 dpe 大量定植，并与 Lactobacillus helsingborgensis 一起显著减少。Commensalibacter 和 Bifidobacterium 在 25 dpe 显著减少，Bacteroides、Escherichia-Shigella 和 Porphyromonadaceae 在 19 到 25 dpe 之间显著减少。我们的研究结果揭示了工蜂从出生到衰老过程中肠道微生物组的演替，为进一步探索肠道微生物组在不同发育阶段的作用提供了理论依据。
