Elsayed Elgarawany Ghada, Abdou Asmaa Gaber, Maher Taie Doha, Motawea Shaimaa Mohammed
Medical Physiology Departments, Faculty of Medicine, Menoufia University, Shebein El-Kom, Egypt.
Pathology Department, Menoufia University, Shebein El-Kom, Egypt.
J Immunoassay Immunochem. 2020;41(1):84-96. doi: 10.1080/15321819.2019.1692029. Epub 2019 Nov 18.
Acetaminophen is a common analgesic-antipyretic agent, which is safe in therapeutic doses but in higher doses can produce hepatic necrosis. The aim of this study is to investigate the hepatoprotective effects of artichoke, silymarin, and both agents in acetaminophen-induced liver damage in mice. Forty male mice were divided into five main groups, (1) control (2) Acetaminophen (APAP) (3) Artichoke leaf extracts (ALE) and APAP (4) silymarin and APAP group (5) ALE, silymarin and APAP groups. Blood samples were collected for the measurement of liver enzymes (ALT, AST, and ALP). The liver was excised, weighed and dissected into two parts, one used for measurement of malondialdehyde (MDA) and glutathione reductase, and the other part used for histopathological examination and assessment of proliferative cell nuclear antigen (PCNA) immunohistochemical expression. APAP group showed a significant increase in liver weight, ALT, AST, ALP, MDA, and PCNA expression with a significant decrease in glutathione reductase in comparison to control group. All these parameters were significantly improved in the three treated groups when compared to APAP group. APAP group showed marked portal inflammation and parenchyma necrosis. Co-administration of ALE and/or silymarin to acetaminophen treated mice showed a significant reduction in PCNA expression compared to APAP group. Both ALE and silymarin co-treatment showed a significant decrease in PCNA percentage to a level near to control group. Artichoke and/or silymarin are suggested to protect against acetaminophen-induced hepatotoxicity in mice by ameliorating liver enzymes, antioxidant effect, decreasing liver damage and proliferation. ALT, alanine transaminase. AST, aspartate transaminase. ALP, alkaline phosphatase.MDA, malondialdehyde. PCNA, proliferative cell nuclear antigen.
对乙酰氨基酚是一种常见的解热镇痛药，治疗剂量下安全，但高剂量时可导致肝坏死。本研究旨在探讨洋蓟、水飞蓟宾以及二者联用对乙酰氨基酚诱导的小鼠肝损伤的保护作用。40只雄性小鼠分为5个主要组，（1）对照组（2）对乙酰氨基酚（APAP）组（3）洋蓟叶提取物（ALE）与APAP组（4）水飞蓟宾与APAP组（5）ALE、水飞蓟宾与APAP组。采集血样检测肝酶（ALT、AST和ALP）。切除肝脏，称重并分成两部分，一部分用于检测丙二醛（MDA）和谷胱甘肽还原酶，另一部分用于组织病理学检查和增殖细胞核抗原（PCNA）免疫组化表达评估。与对照组相比，APAP组肝重量、ALT、AST、ALP、MDA和PCNA表达显著增加，谷胱甘肽还原酶显著降低。与APAP组相比，三个治疗组的所有这些参数均有显著改善。APAP组显示明显的门脉炎症和实质坏死。与APAP组相比，对乙酰氨基酚处理的小鼠联用ALE和/或水飞蓟宾后PCNA表达显著降低。ALE和水飞蓟宾联合处理均使PCNA百分比显著降低至接近对照组水平。提示洋蓟和/或水飞蓟宾可通过改善肝酶水平、抗氧化作用、减轻肝损伤和增殖来预防对乙酰氨基酚诱导的小鼠肝毒性。ALT，丙氨酸转氨酶。AST，天冬氨酸转氨酶。ALP，碱性磷酸酶。MDA，丙二醛。PCNA，增殖细胞核抗原。
