白藜芦醇通过抑制非致病性葡萄球菌的生长来改善 BALB/c 小鼠的肝纤维化。
Resveratrol ameliorates liver fibrosis induced by nonpathogenic Staphylococcus in BALB/c mice through inhibiting its growth.
机构信息
Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450001, Henan Province, China.
Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, 450001, Henan Province, China.
出版信息
Mol Med. 2022 May 4;28(1):52. doi: 10.1186/s10020-022-00463-y.
BACKGROUND
The altered gut microbiota is implicated in the pathogenesis of liver fibrosis. Resveratrol is a candidate for the treatment of liver fibrosis, which could ameliorate the dysregulation of gut microbiota in mice. This study aimed to clarify the role and mechanism of resveratrol in gut microbiota during liver fibrosis.
METHODS
A mouse model of liver fibrosis induced by CCl was conducted to assess the effect of resveratrol on liver fibrosis. The changes of gut microbiota in liver fibrotic mice after resveratrol intervention were assessed using 16S ribosomal RNA sequencing. The mechanism of the gut microbiota dysregulation in liver fibrosis was investigated by Sirius red staining, immunohistochemical assay, bacterial translocation (BT), EUB338 fluorescence in situ hybridization, immunofluorescence, trans-epithelial electrical resistance analysis and paracellular permeability analysis.
RESULTS
Resveratrol relieved CCl-induced liver fibrosis. Besides, resveratrol restrained the gut microbiota Staphylococcus_lentus and Staphylococcus_xylosus in the liver fibrotic mice, and the Staphylococcus_xylosus and Staphylococcus_lentus facilitated the occurrence of BT and the cultures of them enhanced the permeability of intestine. The in vivo assay corroborated that the excessive Staphylococcus_xylosus and Staphylococcus_lentus canceled the protecting effect of resveratrol on liver fibrosis, and Staphylococcus_xylosus or Staphylococcus_lentus alone had a limited impact on the liver injury of normal mice.
CONCLUSION
Resveratrol ameliorated liver fibrosis by restraining the growth of Staphylococcus_xylosus and Staphylococcus_lentus.
背景
肠道微生物群的改变与肝纤维化的发病机制有关。白藜芦醇是治疗肝纤维化的候选药物，它可以改善小鼠肠道微生物群的失调。本研究旨在阐明白藜芦醇在肝纤维化过程中对肠道微生物群的作用和机制。
方法
采用 CCl 诱导的小鼠肝纤维化模型，评估白藜芦醇对肝纤维化的影响。采用 16S 核糖体 RNA 测序评估白藜芦醇干预后肝纤维化小鼠肠道微生物群的变化。通过天狼星红染色、免疫组织化学检测、细菌易位（BT）、EUB338 荧光原位杂交、免疫荧光、跨上皮电阻分析和细胞旁通透性分析，研究肝纤维化中肠道微生物群失调的机制。
结果
白藜芦醇缓解了 CCl 诱导的肝纤维化。此外，白藜芦醇抑制了肝纤维化小鼠的肠道微生物群葡萄球菌属和藤黄微球菌，而藤黄微球菌和葡萄球菌属促进了 BT 的发生，它们的培养物增强了肠道的通透性。体内实验证实，过量的藤黄微球菌和葡萄球菌属取消了白藜芦醇对肝纤维化的保护作用，而单独的藤黄微球菌或葡萄球菌属对正常小鼠的肝损伤影响有限。
结论
白藜芦醇通过抑制藤黄微球菌和葡萄球菌属的生长来改善肝纤维化。
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