Instituto de Carboquímica, ICB-CSIC, Miguel Luesma Castán 4, 50018 Zaragoza, Spain.
Departamento de Farmacología y Fisiología, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet, 177, Zaragoza, 50013, Spain; Instituto de Investigación Sanitaria de Aragón (IIS), Zaragoza, Spain; Instituto Agroalimentario de Aragón - IA2 -, (Universidad de Zaragoza - CITA), Zaragoza, Spain.
Colloids Surf B Biointerfaces. 2020 Jan 1;185:110612. doi: 10.1016/j.colsurfb.2019.110612. Epub 2019 Nov 9.
Given the potential applications of fluorescent carbon nanoparticles in biomedicine, the relationship between their chemical structure, optical properties and biocompatibility has to be investigated in detail. In this work, different types of fluorescent carbon nanoparticles are synthesized by acid treatment, sonochemical treatment, electrochemical cleavage and polycondensation. The particle size ranges from 1 to 6 nm, depending on the synthesis method. Nanoparticles that were prepared by acid or sonochemical treatments from graphite keep a crystalline core and can be classified as graphene quantum dots. The electrochemically produced nanoparticles do not clearly show the graphene core, but it is made of heterogeneous aromatic structures with limited size. The polycondensation nanoparticles do not have CC double bonds. The type of functional groups on the carbon backbone and the optical properties, both absorbance and photoluminescence, strongly depend on the nanoparticle origin. The selected types of nanoparticles are compatible with human intestinal cells, while three of them also show activity against colon cancer cells. The widely different properties of the nanoparticle types need to be considered for their use as diagnosis markers and therapeutic vehicles, specifically in the digestive system.
鉴于荧光碳纳米粒子在生物医药中的潜在应用，必须详细研究它们的化学结构、光学性质和生物相容性之间的关系。在这项工作中，通过酸处理、超声化学处理、电化学裂解和缩聚反应合成了不同类型的荧光碳纳米粒子。根据合成方法的不同，粒径范围为 1 至 6nm。由石墨经酸或超声处理制备的纳米粒子保持结晶核，可归类为石墨烯量子点。电化学产生的纳米粒子没有明显显示出石墨烯核，但它由具有有限尺寸的不均匀芳香结构组成。缩合纳米粒子没有 CC 双键。碳主链上的官能团类型和光吸收和光致发光的光学性质强烈依赖于纳米粒子的来源。所选类型的纳米粒子与人肠细胞兼容，其中三种对结肠癌细胞也具有活性。在将这些纳米粒子用作诊断标记物和治疗载体，特别是在消化系统中时，需要考虑它们广泛的性质差异。
