心肌梗死后炎症的功能意义通过 F-氟脱氧葡萄糖成像在猪模型中进行评估。

Functional significance of post-myocardial infarction inflammation evaluated by F-fluorodeoxyglucose imaging in swine model.

机构信息

Department of Nuclear Medicine, Beijing Chaoyang Hospital, Capital Medical University, 8th Gongtinanlu Rd, Chaoyang District, Beijing, 100020, China.

Department of Nuclear Medicine, The Third Affiliated Hospital of Soochow University, No. 185, Juqian Street, Changzhou, 213003, Jiangsu, China.

出版信息

J Nucl Cardiol. 2020 Apr;27(2):519-531. doi: 10.1007/s12350-019-01952-0. Epub 2019 Nov 18.

DOI:10.1007/s12350-019-01952-0

PMID:31741330

Abstract

BACKGROUND

The aim of the study was to investigate the relationship between post-myocardial infarction (MI) inflammation and left ventricular (LV) remodeling in a swine model by F-fluorodeoxyglucose (FDG) imaging.

METHODS

MI was induced in swine by balloon occlusion of the left anterior descending coronary artery. A series of FDG positron emission tomography (PET) images were taken within 2 weeks post-MI, employing a comprehensive strategy to suppress the physiological uptake of cardiomyocytes. Echocardiography was applied to evaluate LV volume, global and regional function. CD68 macrophage and glucose transporters (GLUT-1, -3 and -4) were investigated by immunostaining.

RESULTS

The physiological uptake of myocardium was adequately suppressed in 92.3% of PET scans verified by visual analysis, which was further confirmed by the minimal expression of myocardial GLUT-4. Higher FDG uptake was observed in the infarct than in the remote area and persisted within the observational period of 2 weeks. The FDG uptake of infarcted myocardium on day 1 post-MI was correlated with LV global remodeling, and the FDG uptake of infarcted myocardium on days 1 and 8 post-MI had a trend of correlating with regional remodeling of the infarct area.

CONCLUSIONS

We here report a feasible swine model for investigating post-MI inflammation. FDG signal in the infarct area of swine persisted for a longer duration than has been reported in small animals. FDG activity in the infarct area could predict LV remodeling.

摘要

背景

本研究旨在通过 F-氟脱氧葡萄糖（FDG）成像探讨心肌梗死后（MI）炎症与左心室（LV）重构的关系。

方法

通过球囊阻塞左前降支冠状动脉在猪模型中诱导 MI。在 MI 后 2 周内采用综合策略抑制心肌细胞的生理摄取，进行一系列 FDG 正电子发射断层扫描（PET）成像。采用超声心动图评估 LV 容积、整体和局部功能。通过免疫染色研究 CD68 巨噬细胞和葡萄糖转运体（GLUT-1、-3 和 -4）。

结果

通过视觉分析证实，92.3%的 PET 扫描中充分抑制了心肌的生理摄取，进一步通过心肌 GLUT-4 的最小表达得到证实。梗死区的 FDG 摄取高于远隔区，并在 2 周的观察期内持续存在。MI 后第 1 天梗死心肌的 FDG 摄取与 LV 整体重构相关，MI 后第 1 天和第 8 天梗死心肌的 FDG 摄取与梗死区的局部重构呈趋势相关。