Wang Su, Gao Jian, Wang Maohuai, Chen Liquan, Zhang Xiaowei, Wang Xiaoyu
Department of Gynecology and Obstetrics, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Department of General Surgery, Panyu Central Hospital, Guangzhou, China.
J Int Med Res. 2019 Dec;47(12):6303-6314. doi: 10.1177/0300060519873472. Epub 2019 Nov 19.
This study investigated the effect of recombinant human connective tissue growth factor (hCTGF) on rat adipose-derived stem cells (ADSCs) and explored the feasibility of using ADSCs to treat pelvic organ prolapse.
ADSCs were isolated from rat inguinal adipose tissue and characterized by flow cytometry and for osteogenic and adipogenic differentiation. ADSCs were treated with recombinant hCTGF and qRT-PCR was performed to detect collagen I and III expression on post-treatment days 7, 14, and 28. Osteogenic and adipogenic differentiation of ADSCs was performed to evaluate the effect of hCTGF. ADSCs were seeded in biological grafting materials, acellular porcine pericardium (APP) and acellular bovine pericardium (ABP), then implanted in the rat vagina. Histology was performed to observe inflammation among different groups.
Collagen I and III expression in ADSCs was significantly increased, and the ability to differentiate into osteogenic and adipogenic lineages was diminished after hCTGF treatment. APP and ABP seeded with ADSCs significantly decreased inflammation and protected from degradation compared with APP and ABP only; ABP seeded with ADSCs had the lowest inflammation.
hCTGF regulates collagen I and III expression and induces ADSC differentiation . ADSCs decrease inflammation associated with APP and ABP .
本研究调查重组人结缔组织生长因子（hCTGF）对大鼠脂肪来源干细胞（ADSCs）的影响，并探索使用ADSCs治疗盆腔器官脱垂的可行性。
从大鼠腹股沟脂肪组织中分离ADSCs，并通过流式细胞术以及成骨和成脂分化进行鉴定。用重组hCTGF处理ADSCs，并在处理后第7、14和28天进行qRT-PCR检测I型和III型胶原蛋白的表达。对ADSCs进行成骨和成脂分化以评估hCTGF的作用。将ADSCs接种于生物移植材料、脱细胞猪心包（APP）和脱细胞牛心包（ABP）中，然后植入大鼠阴道。进行组织学检查以观察不同组之间的炎症情况。
hCTGF处理后，ADSCs中I型和III型胶原蛋白的表达显著增加，且向成骨和成脂谱系分化的能力减弱。与仅使用APP和ABP相比，接种ADSCs的APP和ABP显著减轻了炎症并防止降解；接种ADSCs的ABP炎症最低。
hCTGF调节I型和III型胶原蛋白的表达并诱导ADSC分化。ADSCs减轻与APP和ABP相关的炎症。
