Rothstein N, Stoller T J, Rajan T V
Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461.
Parasitology. 1988 Aug;97 ( Pt 1):75-9. doi: 10.1017/s0031182000066750.
We have determined the molar content of guanine + cytosine (GC content) of DNA of the filarial nematode (Brugia malayi, Brugia pahangi and Dirofilaria imitis) and of the free-living soil nematodes Caenorhabditis elegans and have analysed the DNA for the presence of methylcytosine. Two independent methods, thermal denaturation and direct analysis of base content by HPLC following enzymatic hydrolysis, reveal that the GC content of filarial nematodes is 26-28%. We have been unable to find methylcytosine in the DNA of B. malayi.
我们已经测定了丝虫线虫（马来布鲁线虫、彭亨布鲁线虫和犬恶丝虫）以及自由生活的土壤线虫秀丽隐杆线虫DNA中鸟嘌呤 + 胞嘧啶的摩尔含量（GC含量），并分析了DNA中甲基胞嘧啶的存在情况。两种独立的方法，即热变性法和酶解后通过高效液相色谱直接分析碱基含量，结果显示丝虫线虫的GC含量为26 - 28%。我们在马来布鲁线虫的DNA中未能发现甲基胞嘧啶。
