Mendis A H, Rose M E, Rees H H, Goodwin T W
Mol Biochem Parasitol. 1983 Nov;9(3):209-26. doi: 10.1016/0166-6851(83)90098-1.
Adult males and females of the dog heartworm, Dirofilaria immitis, were extracted separately and, following separation of the free and conjugated ecdysteroid fractions, the conjugates were hydrolysed enzymically. Both the ecdysteroids released by hydrolysis of the conjugates and the free hormones were further purified and analysed by a combination of radioimmunoassay, thin-layer chromatography and high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by gas-liquid chromatography/mass spectrometry (selected ion monitoring). Both males and females contained free and conjugated ecdysteroids. Evidence was obtained for the presence of ecdysone, 20-hydroxyecdysone, 20,26-dihydroxyecdysone and possibly ponasterone A. The possible parallel between ecdysteroid endocrinology in nematodes and insects is discussed.
犬心丝虫（恶丝虫）的成年雄性和雌性虫体被分别提取出来，在游离和结合蜕皮甾体组分分离之后，结合物进行酶促水解。结合物水解释放出的蜕皮甾体以及游离激素均进一步纯化，并通过放射免疫测定、薄层色谱和高效液相色谱（通过放射免疫测定监测组分）以及气液色谱/质谱联用（选择离子监测）进行分析。雄性和雌性虫体均含有游离和结合的蜕皮甾体。已获得证据表明存在蜕皮激素、20-羟基蜕皮激素、20,26-二羟基蜕皮激素以及可能存在的波那甾酮A。本文还讨论了线虫和昆虫蜕皮甾体内分泌学之间可能存在的相似之处。
