CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, 264003, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai, 264003, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, PR China.
Environ Pollut. 2020 Feb;257:113591. doi: 10.1016/j.envpol.2019.113591. Epub 2019 Nov 9.
Cadmium (Cd) is an important heavy metal pollutant in the Bohai Sea. Mitochondria are recognized as the key target for Cd toxicity. However, mitochondrial responses to Cd have not been fully investigated in marine fishes. In this study, the mitochondrial responses were characterized in gills of juvenile flounder Paralichthys olivaceus treated with two environmentally relevant concentrations (5 and 50 μg/L) of Cd for 14 days by determination of mitochondrial membrane potential (MMP), observation of mitochondrial morphology and quantitative proteomic analysis. Both Cd treatments significantly decreased MMPs of mitochondria from flounder gills. Mitochondrial morphologies were altered in Cd-treated flounder samples, indicated by more and smaller mitochondria. iTRAQ-based proteomic analysis indicated that a total of 128 proteins were differentially expressed in both Cd treatments. These proteins were basically involved in various biological processes in gill mitochondria, including mitochondrial morphology and import, tricarboxylic acid (TCA) cycle, oxidative phosphorylation (OXPHOS), primary bile acid biosynthesis, stress resistance and apoptosis. These results indicated that dynamic regulations of energy homeostasis, cholesterol metabolism, stress resistance, apoptosis, and mitochondrial morphology in gill mitochondria might play significant roles in response to Cd toxicity. Overall, this study provided a global view on mitochondrial toxicity of Cd in flounder gills using iTRAQ-based proteomics.
镉 (Cd) 是渤海的一种重要重金属污染物。线粒体被认为是镉毒性的关键靶标。然而，海洋鱼类中线粒体对镉的反应尚未得到充分研究。在这项研究中，通过测定线粒体膜电位 (MMP)、观察线粒体形态和定量蛋白质组学分析，研究了 Cd 处理 14 天后牙鲆幼鱼鳃中的线粒体反应。两种 Cd 处理均显著降低了牙鲆鳃中线粒体的 MMP。Cd 处理的牙鲆样本中的线粒体形态发生改变，表现为更多和更小的线粒体。iTRAQ 基于蛋白质组学分析表明，两种 Cd 处理共鉴定到 128 种差异表达蛋白。这些蛋白主要参与了鳃中线粒体的各种生物学过程，包括线粒体形态和输入、三羧酸 (TCA) 循环、氧化磷酸化 (OXPHOS)、初级胆汁酸生物合成、应激反应和细胞凋亡。这些结果表明，能量稳态、胆固醇代谢、应激反应、细胞凋亡和线粒体形态的动态调节可能在牙鲆鳃对镉毒性的反应中发挥重要作用。总的来说，本研究使用 iTRAQ 蛋白质组学方法为牙鲆鳃中镉的线粒体毒性提供了全面的认识。
