Department of Parasitology and Genetics, Institute for Medical Science, Kosin University College of Medicine, Busan, Republic of Korea.
Pathology Research Division, National Institute of Fisheries Science, Busan, Republic of Korea.
Genes Genomics. 2021 Jul;43(7):701-712. doi: 10.1007/s13258-021-01097-w. Epub 2021 Apr 13.
Olive flounder (Paralichthys olivaceus) is one of the major cultured fish species in Asia including Korea. However, the mass mortality of olive flounder caused by various pathogens leads to huge economic loss. The pathogens that lead to fish mortality include parasites, bacteria, and viruses that can cause various kinds of diseases.
The purpose of this study was to investigate the protein expression patterns in the gills and spleens of olive flounder after artificial infection. We hypothesized that proteomics levels in gills and spleen may be differentially expressed depending on infectious agents.
To investigate the expression pattern of proteins in gills and spleens, olive flounders were experimentally infected with VHSV (virus), S. parauberis (bacteria), or M. avidus (pathogenic ciliate). Proteins were extracted from the gills and spleens of infected olive flounder. We used 2-DE analysis with LC-MS/MS to investigate proteome changes in infected olive flounders.
The results of the LC-MS/MS analyses showed different protein expression profiles depending on pathogenic sources and target organs. Proteins related to cytoskeletal structure like keratin, calmodulin and actin were mostly expressed in the infected gills. Proteins involved in the metabolism pathway like glycolysis were expressed mainly in the spleens. The protein profiles of S. parauberis and VHSV infection groups had many similarities, but the profile of the M. avidus infection group was greatly different in the gill and spleen.
Our results indicate that measures according to the characteristics of each pathogen are necessary for disease prevention and treatment of farmed fish.
牙鲆（Paralichthys olivaceus）是亚洲（包括韩国）主要养殖鱼类之一。然而，各种病原体导致的牙鲆大规模死亡导致了巨大的经济损失。导致鱼类死亡的病原体包括寄生虫、细菌和病毒，它们可以引起各种疾病。
本研究旨在调查人工感染后牙鲆鳃和脾脏的蛋白质表达模式。我们假设鳃和脾脏中的蛋白质组水平可能因感染因子而异。
为了研究鳃和脾脏中蛋白质的表达模式，我们用 VHSV（病毒）、S. parauberis（细菌）或 M. avidus（致病纤毛虫）对牙鲆进行了人工感染。从感染牙鲆的鳃和脾脏中提取蛋白质。我们使用 2-DE 分析与 LC-MS/MS 来研究感染牙鲆的蛋白质组变化。
LC-MS/MS 分析的结果表明，根据病原体来源和靶器官的不同，蛋白质表达谱也不同。与细胞骨架结构相关的蛋白质，如角蛋白、钙调蛋白和肌动蛋白，在感染的鳃中表达最多。参与代谢途径的蛋白质，如糖酵解，主要在脾脏中表达。S. parauberis 和 VHSV 感染组的蛋白质谱有许多相似之处，但 M. avidus 感染组在鳃和脾脏中的蛋白质谱则大不相同。
我们的结果表明，针对每种病原体的特点采取措施对养殖鱼类的疾病预防和治疗是必要的。
