Department of Psychiatry and Behavioral Neurosciences, McMaster University, Hamilton, Ontario, Canada.
Department of Medicine, Division of Neurology, McMaster University, Hamilton, Ontario, Canada.
J Stroke Cerebrovasc Dis. 2020 Jan;29(1):104502. doi: 10.1016/j.jstrokecerebrovasdis.2019.104502. Epub 2019 Nov 16.
Cerebral dopamine neurotrophic factor plays a critical role in repairing and maintaining healthy neurons in pathological conditions such as stroke. However, the association between cerebral dopamine neurotrophic factor expression and stroke has only recently been investigated in preclinical models and is rarely described in human studies.
The aims of this were to examine neurological alterations mirrored in human blood platelet cerebral dopamine neurotrophic factor gene expression. Cerebral dopamine neurotrophic factor is expressed in both the central nervous system and peripheral blood. Blood platelets are often used to model neuronal behavior because they exhibit biochemical impairments similar to brain tissues of patients with neurological disorders.
RNA was isolated from platelets and cDNA was synthesized to quantify cerebral dopamine neurotrophic factor gene expression of 36 stroke patients compared to 72 healthy aged-matched controls through real-time PCR. Further grouping analyses of data with regard to age, sex, and medication history were performed.
Cerebral dopamine neurotrophic factor gene expression was significantly reduced in stroke patients relative to control subjects (P = .013). Subsequent analysis revealed a significant difference in expression between males and females within the control group (P = .026). Decreased cerebral dopamine neurotrophic factor expression was only observed in male stroke patients compared to their sex-matched controls (P = .008). Grouping stroke patients based on their medication history did not significantly alter cerebral dopamine neurotrophic factor gene expression.
Further studies investigating cerebral dopamine neurotrophic factor expression could be directed towards the interplay of the central nervous system, hematopoietic derivatives, and utilizing cerebral dopamine neurotrophic factor as a therapeutic tool.
脑源性神经营养因子在修复和维持病理状态下（如中风）的健康神经元方面起着关键作用。然而，脑源性神经营养因子表达与中风之间的关联仅在最近的临床前模型中进行了研究，在人类研究中很少描述。
本研究旨在检测人类血小板脑源性神经营养因子基因表达中反映的神经学改变。脑源性神经营养因子在中枢神经系统和外周血液中均有表达。血小板常被用于模拟神经元行为，因为它们表现出与患有神经障碍的患者脑组织相似的生化损伤。
通过实时 PCR 从血小板中分离 RNA 并合成 cDNA，以定量 36 名中风患者和 72 名健康年龄匹配对照者的脑源性神经营养因子基因表达。进一步针对年龄、性别和用药史对数据进行分组分析。
与对照组相比，中风患者的脑源性神经营养因子基因表达显著降低（P=0.013）。进一步分析显示，对照组内男女之间的表达存在显著差异（P=0.026）。与女性对照组相比，仅在男性中风患者中观察到脑源性神经营养因子表达降低（P=0.008）。基于用药史对中风患者进行分组，并未显著改变脑源性神经营养因子基因表达。
进一步研究脑源性神经营养因子表达可以针对中枢神经系统、造血衍生物之间的相互作用，并将脑源性神经营养因子作为一种治疗工具。
