Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 PLUS Project, Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Korea.
Cell Prolif. 2020 Jan;53(1):e12729. doi: 10.1111/cpr.12729. Epub 2019 Nov 19.
The successional dental lamina is the distinctive structure on the lingual side of the vertebrate tooth germ. The aim of this study was to investigate the relationship among Sox2, Claudin10 and laminin5 and the role of Sox2 in successional dental lamina proliferation during vertebrate tooth development.
To understand the successional dental lamina, two types of successional tooth formation, that in geckos (with multiple rounds of tooth generation) and that in mice (with only one round of tooth generation), were analysed.
Unique coexpression patterns of Sox2 and Claudin10 expression were compared in the successional dental lamina from the cap stage to the late bell stage in the mouse tooth germ and in juvenile gecko teeth to support continuous tooth replacement. Furthermore, Laminin5 expression was shown in the cap stage and decreased after the bell stage. Upon comparing the epithelial cell cycles and cell proliferation in successional dental lamina regions between mouse and gecko molars using BrdU and IdU staining and pulse-chase methods, distinctive patterns of continuous expression were revealed. Moreover, Sox2 overexpression with a lentiviral system resulted in hyperplastic dental epithelium in mouse molars.
Our findings indicate that the regulation of Sox2 in dental lamina proliferation is fundamental to the successional dental lamina in both species.
连续的牙板是脊椎动物牙胚舌侧的特有结构。本研究旨在探讨 Sox2、Claudin10 和层粘连蛋白 5 之间的关系，以及 Sox2 在脊椎动物牙齿发育过程中对连续牙板增殖的作用。
为了了解连续的牙板，分析了两种类型的连续牙齿形成，即壁虎（具有多轮牙齿生成）和小鼠（仅具有一轮牙齿生成）。
在小鼠牙胚的帽状期到晚期钟状期的连续牙板中，比较了 Sox2 和 Claudin10 表达的独特共表达模式，并支持连续牙齿替换。此外，在帽状期表达层粘连蛋白 5，并在钟状期后减少。通过使用 BrdU 和 IdU 染色和脉冲追踪方法比较小鼠和壁虎磨牙中连续牙板区域的上皮细胞周期和细胞增殖，揭示了连续表达的独特模式。此外，利用慢病毒系统过表达 Sox2 导致小鼠磨牙中牙上皮过度增生。
我们的研究结果表明，Sox2 在牙板增殖中的调节对两种物种的连续牙板都至关重要。
