Daher K A, Lehrer R I, Ganz T, Kronenberg M
University of California, Department of Microbiology and Immunology, Los Angeles 90024.
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7327-31. doi: 10.1073/pnas.85.19.7327.
Four clones that encode defensins, a group of microbicidal and cytotoxic peptides made by neutrophils, were isolated from an HL-60 human promyelocytic leukemia cDNA library. Analysis of these clones indicated that the defensins are made as precursor proteins, which must be cleaved to yield the mature peptides. Defensin mRNA was detected in normal bone marrow cells, but not in normal peripheral blood leukocytes. Defensin transcripts were also found in the peripheral leukocytes of some leukemia patients and in some lung and intestine tissues. Defensin mRNA content was augmented by treatment of HL-60 cells with dimethyl sulfoxide. These results define important aspects of the mechanism of synthesis and the tissue-specific expression of a major group of neutrophil granule proteins.
从HL-60人早幼粒细胞白血病cDNA文库中分离出四个编码防御素的克隆，防御素是一组由中性粒细胞产生的具有杀菌和细胞毒性的肽。对这些克隆的分析表明，防御素是以前体蛋白的形式产生的，必须经过切割才能产生成熟的肽。在正常骨髓细胞中检测到防御素mRNA，但在正常外周血白细胞中未检测到。在一些白血病患者的外周白细胞以及一些肺和肠组织中也发现了防御素转录本。用二甲基亚砜处理HL-60细胞可增加防御素mRNA的含量。这些结果确定了一组主要的中性粒细胞颗粒蛋白的合成机制和组织特异性表达的重要方面。
