修饰蛋白质上预先选定的位点：肌球蛋白重链的633 - 642位残基片段是肌动蛋白结合位点的一部分。
Modifying preselected sites on proteins: the stretch of residues 633-642 of the myosin heavy chain is part of the actin-binding site.
作者信息
Chaussepied P, Morales M F
机构信息
Cardiovascular Research Institute, University of California, San Francisco 94143.
出版信息
Proc Natl Acad Sci U S A. 1988 Oct;85(20):7471-5. doi: 10.1073/pnas.85.20.7471.
Abstract
We have designed an "antipeptide" capable of firmly and specifically interacting with a preselected stretch of myosin S-1 heavy chain. Covalent attachment of this antipeptide to its target stretch, residues 633-642, does not affect the intrinsic ATPase activities of the protein but significantly reduces the actin-binding capabilities of the myosin head.
摘要
我们设计了一种“抗肽”，它能够与肌球蛋白S-1重链预先选定的一段序列牢固且特异性地相互作用。将这种抗肽共价连接到其目标序列（第633 - 642位残基）上，不会影响该蛋白质固有的ATP酶活性，但会显著降低肌球蛋白头部与肌动蛋白的结合能力。
Zotero 插件