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修饰蛋白质上预先选定的位点：肌球蛋白重链的633 - 642位残基片段是肌动蛋白结合位点的一部分。

Modifying preselected sites on proteins: the stretch of residues 633-642 of the myosin heavy chain is part of the actin-binding site.

作者信息

Chaussepied P, Morales M F

机构信息

Cardiovascular Research Institute, University of California, San Francisco 94143.

出版信息

Proc Natl Acad Sci U S A. 1988 Oct;85(20):7471-5. doi: 10.1073/pnas.85.20.7471.

DOI:10.1073/pnas.85.20.7471
PMID:3174648
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC282213/
Abstract

We have designed an "antipeptide" capable of firmly and specifically interacting with a preselected stretch of myosin S-1 heavy chain. Covalent attachment of this antipeptide to its target stretch, residues 633-642, does not affect the intrinsic ATPase activities of the protein but significantly reduces the actin-binding capabilities of the myosin head.

摘要

我们设计了一种“抗肽”，它能够与肌球蛋白S-1重链预先选定的一段序列牢固且特异性地相互作用。将这种抗肽共价连接到其目标序列（第633 - 642位残基）上，不会影响该蛋白质固有的ATP酶活性，但会显著降低肌球蛋白头部与肌动蛋白的结合能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/282213/7d7b1db9afc8/pnas00299-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/282213/7d7b1db9afc8/pnas00299-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6546/282213/7d7b1db9afc8/pnas00299-0058-a.jpg

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Proc Natl Acad Sci U S A. 1988 Oct;85(20):7471-5. doi: 10.1073/pnas.85.20.7471.
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Identification of myosin-binding sites on the actin sequence.肌动蛋白序列上肌球蛋白结合位点的鉴定。
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8
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9
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