Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, 350003, China.
Animal Veterinary and Aquatic Product Bureau, Nanping, 353000, China.
Mol Cell Probes. 2020 Feb;49:101489. doi: 10.1016/j.mcp.2019.101489. Epub 2019 Nov 17.
An unclassified calicivirus (CV) detected in geese was recently reported and proposed as a new member of the family Caliciviridae. There is limited information about the epidemiology, etiology and detection method of goose-origin CV (GCV) to date. In this study, an EvaGreen based fluorescence quantitative real-time RT-PCR assay was developed and optimized for the detection of GCVs. The assay sensitively detected GCV RNA template with a good linear standard curve. We also demonstrated the specificity and reproducibility of the detection method for GCVs. Thus, the method developed in this study will benefit the investigation of possible sporadic outbreaks of CV infections in geese, as well as epidemiological and etiological studies of GCVs.
一种在鹅中检测到的未分类杯状病毒（CV）最近被报道，并被提议作为杯状病毒科的一个新成员。迄今为止，关于鹅源杯状病毒（GCV）的流行病学、病因学和检测方法的信息有限。在本研究中，建立并优化了基于 EvaGreen 的荧光实时 RT-PCR 检测方法，用于检测 GCV。该检测方法对 GCV RNA 模板具有良好的线性标准曲线和较高的灵敏度。我们还验证了该检测方法对 GCV 的特异性和重复性。因此，本研究建立的方法将有助于调查鹅中可能发生的 CV 感染散发疫情，以及对 GCV 的流行病学和病因学研究。
