Wang Fumin, Wang Minghang, Dong Yunhan, Zhang Bing, Zhang Dabing
Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People's Republic of China.
Arch Virol. 2017 Jul;162(7):2115-2118. doi: 10.1007/s00705-017-3302-8. Epub 2017 Mar 13.
A novel calicivirus (strain H146) was detected in a goose and sequenced. The H146 genome consisted of two open reading frames (ORFs) with an 8-nucleotide (nt) overlap between the two ORFs, similar to what has been found in the bat sapovirus TLC58. The virus was most closely related to nacoviruses when comparing the complete genome sequence (49% identity), non-structural region (NS; 31-34% amino acid [aa] sequence identity), and major structural VP1 region (28-30% aa identity), whereas both goose calicivirus N and feline calicivirus were the closest relatives of H146 in the VP2 region (20% aa sequence identity). The levels of divergence between H146 and its closest relatives in different genomic regions are comparable to those between some members of different genera. Phylogenetic analysis based on the NS and VP1 amino acid sequences clearly demonstrated that H146 formed a separate clade. Thus, calicivirus H146 was identified as a founding member of a novel genus for which we propose the name "Sanovirus".
在一只鹅体内检测到一种新型杯状病毒（H146株）并进行了测序。H146基因组由两个开放阅读框（ORF）组成，两个ORF之间有8个核苷酸（nt）的重叠，这与在蝙蝠札幌病毒TLC58中发现的情况相似。在比较完整基因组序列（49%的同一性）、非结构区（NS；31 - 34%的氨基酸[aa]序列同一性）和主要结构VP1区（28 - 30%的aa同一性）时，该病毒与纳科病毒关系最为密切，而鹅杯状病毒N和猫杯状病毒在VP2区（20%的aa序列同一性）是H146最亲近的亲属。H146与其在不同基因组区域的最亲近亲属之间的差异水平与不同属的一些成员之间的差异水平相当。基于NS和VP1氨基酸序列的系统发育分析清楚地表明，H146形成了一个单独的进化枝。因此，杯状病毒H146被鉴定为一个新属的创始成员，我们提议将其命名为“沙诺病毒属”。
