Ohishi Kazue, Amano Masao, Nakamatsu Ken, Miyazaki Nobuyuki, Tajima Yuko, Yamada Tadasu K, Matsuda Ayaka, Ochiai Mari, Matsuishi Takashi F, Taru Hajime, Iwao Hajime, Maruyama Tadashi
Faculty of Engineering, Tokyo Polytechnic University, 1583 Iiyama, Atsugi, Kanagawa 243-0297, Japan.
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
J Vet Med Sci. 2020 Jan 10;82(1):43-46. doi: 10.1292/jvms.19-0481. Epub 2019 Nov 19.
A serologic investigation of Brucella infection was performed in 7 species of cetaceans inhabiting along the coast of Japan. A total of 32 serum samples were examined by enzyme-linked immunosorbent assay (ELISA) using Brucella abortus and B. canis antigens. One serum sample from five melon-headed whales (Peponocephala electra) was positive for B. abortus. No serum sample showed positive for B. canis. The ELISA-positive melon-headed whale serum demonstrated a strong band appearance only against B. abortus antigens in Western blot analysis. Many detected bands were discrete, while some of them had a smeared appearance. The present results indicate that Brucella infection occurred in melon-headed whale population and the bacterial antigenicity is more similar to that of B. abortus than B. canis.
对栖息在日本沿海的7种鲸类动物进行了布鲁氏菌感染的血清学调查。使用流产布鲁氏菌和犬布鲁氏菌抗原，通过酶联免疫吸附测定（ELISA）对总共32份血清样本进行了检测。来自5头瓜头鲸（Peponocephala electra）的一份血清样本对流产布鲁氏菌呈阳性。没有血清样本对犬布鲁氏菌呈阳性。ELISA阳性的瓜头鲸血清在蛋白质印迹分析中仅对流产布鲁氏菌抗原显示出强条带外观。检测到的许多条带是离散的，而其中一些有条带拖尾现象。目前的结果表明，瓜头鲸种群中发生了布鲁氏菌感染，并且该细菌的抗原性与流产布鲁氏菌的抗原性比犬布鲁氏菌的更相似。
