Department of Environmental Chemistry, IDAEA-CSIC, 08034, Barcelona, Spain.
Facultad de Ciencias, Universidad Nacional de Educación a Distancia, UNED, Senda del Rey 9, 28040, Madrid, Spain.
Environ Sci Pollut Res Int. 2020 Jan;27(2):1475-1484. doi: 10.1007/s11356-019-06751-1. Epub 2019 Nov 20.
Fingerprinting of the main lipid components of the digestive gland of the Icelandic scallop-Chlamys islandica-has been performed by ultra-high-performance liquid chromatography coupled with time of flight high-resolution mass spectrometry, UHPLC-HRMS/ToF. This method allowed the identification of 224 lipids, including phosphatidylcholines (PC), plasmanyl (PC-O)/plasmenyl (PC-P) phosphatidylcholines, lyso-phosphatidylcholines (LPC), and their plasmanyl/plasmenyl forms (LPC-O/LPC-P). Diacylglycerols (DG), triacylglycerols (TG), and cholesteryl esters (CE) were the neutral lipids (NL) analyzed. While all of the lipids showed a strong seasonal dependence in terms of quantity, only NLs presented significant qualitative changes. Principal component analysis (PCA) of TG and DG profiles evidenced a prevalence of low unsaturated TGs and DGs in spring, which were replaced by species with a higher degree of unsaturations in summer. In autumn, long and highly unsaturated TGs constitute the lipid fraction of the digestive gland of the scallop, while DG species offer a mixed profile. This study contributes to the characterization and the elucidation of the lipidome of Chlamys islandica and provides baseline data for further study of the effects of pollutants on the lipidome of the Icelandic scallop, often used as a sentinel species in biomonitoring programs.
已通过超高效液相色谱-飞行时间高分辨率质谱联用（UHPLC-HRMS/ToF）对冰岛扇贝（Chlamys islandica）消化腺的主要脂质成分进行了指纹图谱分析。该方法共鉴定出 224 种脂质，包括磷脂酰胆碱（PC）、溶血磷脂酰胆碱（LPC）及其酰基化形式（LPC-O/LPC-P）、Plasmanyl（PC-O）/Plasmenyl（PC-P）磷脂酰胆碱。此外，还分析了二酰基甘油（DG）、三酰基甘油（TG）和胆甾醇酯（CE）等中性脂质（NL）。尽管所有脂质的含量均表现出强烈的季节性变化，但只有 NL 存在显著的定性变化。对 TG 和 DG 谱图进行主成分分析（PCA）表明，春季 TG 和 DG 以低不饱和物种为主，夏季则以更高不饱和度的物种为主。秋季，长链和高度不饱和的 TG 构成了扇贝消化腺的脂质部分，而 DG 则呈现混合谱图。本研究为 Chlamys islandica 的脂质组学特征和阐明提供了依据，为进一步研究污染物对冰岛扇贝脂质组的影响提供了基础数据，该扇贝常被用作生物监测计划中的指示物种。
