Llanes Kennesa Klariz R, Besana Cyrelle M, Paller Vachel Gay V
1Animal Biology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, College, Laguna 4031 Philippines.
2Present Address: Department of Biological Sciences, College of Arts and Sciences, University of Southern Mindanao, Kabacan, Cotabato 9407 Philippines.
J Parasit Dis. 2019 Dec;43(4):679-685. doi: 10.1007/s12639-019-01147-x. Epub 2019 Sep 4.
The common dog roundworm can infect other animals and humans which may act as their paratenic and accidental hosts, respectively. Larvae do not further develop to adult worms in these hosts. Instead, they undergo migration to various body organs, causing the neglected parasitic disease known as toxocariasis. Although rats are considered as potential paratenic hosts of the parasite, there are only few studies which utilized (Sprague-Dawley strain) for experimental infections involving toxocariasis. This study aimed to determine whether could establish in Sprague-Dawley rats artificially infected with 500 embryonated eggs and if the animals can be used as animal models for toxocariasis. Following squash method and tissue digestion, larvae were recovered from the lungs, liver and brain of the infected rats. Furthermore, gross examination of organs revealed macroscopic lesions and hemorrhages in the lungs and brain. Microscopically, accumulation of inflammatory cells, thickening of alveoli lining and destruction of bronchial walls and hepatic necrosis were observed. This study showed that has established in Sprague-Dawley rats and could serve as a model for infection studies.
常见的犬蛔虫可感染其他动物和人类，它们分别可能作为犬蛔虫的转续宿主和偶然宿主。幼虫在这些宿主体内不会进一步发育为成虫。相反，它们会迁移到身体的各个器官，引发被称为弓首蛔虫病的被忽视的寄生虫病。尽管大鼠被认为是该寄生虫的潜在转续宿主，但仅有少数研究使用（斯普拉格-道利品系）进行涉及弓首蛔虫病的实验性感染。本研究旨在确定[此处原文可能有缺失信息]能否在人工感染500枚感染性虫卵的斯普拉格-道利大鼠体内定殖，以及这些动物是否可作为弓首蛔虫病的动物模型。采用压片法和组织消化法后，从感染大鼠的肺、肝和脑中回收了幼虫。此外，对器官的大体检查发现肺和脑有宏观病变和出血。在显微镜下，观察到炎症细胞聚集、肺泡壁增厚、支气管壁破坏以及肝坏死。本研究表明[此处原文可能有缺失信息]已在斯普拉格-道利大鼠体内定殖，可作为[此处原文可能有缺失信息]感染研究的模型。
