Sahu Mahesh Chandra, Swain Santosh Kumar, Kar Subrat Kumar
1Directorate of Medical Research, IMS and SUM Hospital, Siksha 'O' Anusandhan (Deemed to be University), K8, Kalinganagar, Bhubaneswar, Odisha 751003 India.
2Department of Otorhinolaryngology, IMS and SUM Hospital, Siksha 'O' Anusandhan (Deemed to be University), K8, Kalinganagar, Bhubaneswar, Odisha 751003 India.
Indian J Otolaryngol Head Neck Surg. 2019 Nov;71(Suppl 2):1300-1308. doi: 10.1007/s12070-018-1358-8. Epub 2018 Apr 13.
is an important chronic suppurative otitis media (CSOM) pathogen that exhibits multiple resistances to antibiotics with increasing frequency, making patient treatment more difficult. The aim of the study is to ascertain the genetically diversity of this clinically isolated with inter simple sequence repeat (ISSR) markers. All 25 were isolated from CSOM patients by taking their ear swabs and culturing on blood agar and MacConkey agar. All strains were identified with morphological characters and biochemical testing. The antimicrobial susceptibility testing was carried out according to National Committee for Clinical Laboratory Standards. ISSR was used to study the genetic diversity of . Clinically CSOM isolated 25 were 88% Ciprofloxacin resistant and similarly resistant to other antibiotics were documented. The study has been made using ISSR marker to find out the genomic relation among the strains/populations of . The result was shown that maximum similarity (80%) was between S-11 and S-13 and minimum (28.2%) was between S-4 and S-16 with an average similarity of 53.2%. The dendogram showed a distinct separation in between all the strains/populations of . The strains/populations were broken up into two main clusters in which small one bear two strains/populations (S-4 and S-9) and another cluster have another 23 strains/populations. These 23 strains were also separated to form subcluster by having different range of small clades. The genetically diversity of pathogenic present in CSOM at our hospital indicates the coexistence different strains due to different antibiotic sensitivity patterns. The conventional culture and sensitivity methods are time consuming whereas in PCR, it will detect within 4-6 h for effective antibiotic. Basing upon the banding pattern with ISSR primers, clinicians can prescribe the effective antibiotics accordingly for CSOM patients in the same day.
是一种重要的慢性化脓性中耳炎（CSOM）病原体，对多种抗生素的耐药性日益增加，这使得患者治疗更加困难。本研究的目的是通过简单序列重复区间（ISSR）标记确定这种临床分离株的遗传多样性。通过采集CSOM患者的耳拭子并在血琼脂和麦康凯琼脂上培养，从所有25名患者中分离出该病原体。所有菌株均通过形态特征和生化试验进行鉴定。抗菌药物敏感性试验按照美国国家临床实验室标准委员会的标准进行。使用ISSR研究该病原体的遗传多样性。临床分离的25株CSOM病原体对环丙沙星耐药率为88%，对其他抗生素的耐药情况也有记录。本研究使用ISSR标记来找出该病原体菌株/群体之间的基因组关系。结果表明，S-11和S-13之间的相似度最高（80%），S-4和S-16之间的相似度最低（28.2%），平均相似度为53.2%。树形图显示该病原体的所有菌株/群体之间有明显的分离。菌株/群体被分为两个主要簇，其中较小的一个簇包含两个菌株/群体（S-4和S-9），另一个簇包含另外23个菌株/群体。这23个菌株也通过不同范围的小分支进一步分离形成亚簇。我院CSOM中存在的致病性该病原体的遗传多样性表明，由于不同的抗生素敏感性模式，不同菌株共存。传统的培养和药敏方法耗时较长，而在PCR中，4-6小时内就能检测出有效的抗生素。根据ISSR引物的条带模式，临床医生可以在同一天为CSOM患者相应地开出有效的抗生素。
