Riesco O, Roncoroni A, Smayevsky J, Bianchini H
Departamento de Patología Clínica, Clínicas Dr. Norberto Quirno, Buenos Aires, Argentina.
Rev Argent Microbiol. 1988 Apr-Jun;20(2):103-6.
The ability of Serratia sp. to hydrolyze Tween 80 and the usefulness of this test for differentiating it from other Gram negative bacilli was studied, comparing it with the capacity of production of DNAsa by the same strains using the O-Toluidine blue method. The total number of strains assayed was 88. The sensitivity obtained was 83.9% and specificity 91.3%, with a positive predictive value of 83.9% and a negative predictive value of 91.2%. Using this method to differentiate only non-pigmented Serratia from Klebsiella sp. and Enterobacter sp. the sensitivity and specificity obtained was 100%. Concerning the cost, this method proved to be 3.86 times cheaper than the DNAsa one.
研究了粘质沙雷氏菌水解吐温80的能力以及该试验在将其与其他革兰氏阴性杆菌区分开来方面的实用性,并使用邻甲苯胺蓝法将其与同一菌株产生DNA酶的能力进行比较。检测的菌株总数为88株。获得的灵敏度为83.9%,特异性为91.3%,阳性预测值为83.9%,阴性预测值为91.2%。使用该方法仅区分非色素沉着的粘质沙雷氏菌与克雷伯菌属和肠杆菌属,获得的灵敏度和特异性为100%。在成本方面,该方法被证明比DNA酶法便宜3.86倍。