肠杆菌属和沙雷氏菌属各物种间的DNA相关性

DNA relatedness among species of Enterobacter and Serratia.

作者信息

Steigerwalt A G, Fanning G R, Fife-Asbury M A, Brenner D J

出版信息

Can J Microbiol. 1976 Feb;22(2):121-37. doi: 10.1139/m76-018.

Abstract

Species of Enterobacter and Serratia were examined for deoxyribonucleic acid relatedness to Klebsielleae, to atypical erwiniae, and to other members of Enterobacteriaceae. Deoxyribonucleic acid hybridization and then hydroxyapatite chromatography was the technique used to assess relatedness. Strains of Enterobacter cloacae formed two separate hybridization groups that correlate with the presence or absence of yellow pigment. Pigmented E. cloacae were 75-100% related, but they were only 40-50% related to unpigmented strains. Conversely, unpigmented strains were 70% or more related but were only 40-50% related to the pigmented strains. Both pigmented and unpigmented E. cloacae were 40-45% related to Enterobacter aerogenes and klebsiellae, and 20-30% related to Serratia species and Enterobacter hafniae. Atypical erwiniae were highly related to E. cloacae. Serratia marcescens strains formed one closely related group. Serratia liquefaciens strains formed a single, more disperse, relatedness group, as did isolates of Serratia rubidaea. These species were related throughout a substantial portion of their genomes. A group of lysine-positive "Citrobacter-like" strains were 40-50% related to Serratia species. Only four E. hafniae strains were tested. Two of these were highly related, while the other two were only 50% related to the reference strain. Enterobacter hafniae was only 15-20% related to other Enterobacteriaceae.

摘要

对肠杆菌属和沙雷氏菌属的菌种进行了检测，以确定其与克雷伯氏菌属、非典型欧文氏菌以及肠杆菌科其他成员的脱氧核糖核酸相关性。采用脱氧核糖核酸杂交，然后进行羟基磷灰石层析的技术来评估相关性。阴沟肠杆菌菌株形成了两个独立的杂交组，这与黄色色素的有无相关。有色素的阴沟肠杆菌菌株之间的相关性为75%-100%，但它们与无色素菌株的相关性仅为40%-50%。相反，无色素菌株之间的相关性为70%或更高，但与有色素菌株的相关性仅为40%-50%。有色素和无色素的阴沟肠杆菌与产气肠杆菌和克雷伯氏菌的相关性均为40%-45%，与沙雷氏菌属菌种和哈夫尼亚肠杆菌的相关性为20%-30%。非典型欧文氏菌与阴沟肠杆菌高度相关。粘质沙雷氏菌菌株形成了一个紧密相关的组。液化沙雷氏菌菌株形成了一个单一的、相关性更分散的组，深红沙雷氏菌的分离株也是如此。这些菌种在其基因组的很大一部分中具有相关性。一组赖氨酸阳性的“类柠檬酸杆菌”菌株与沙雷氏菌属菌种的相关性为40%-50%。仅测试了四株哈夫尼亚肠杆菌菌株。其中两株高度相关，而另外两株与参考菌株的相关性仅为50%。哈夫尼亚肠杆菌与其他肠杆菌科的相关性仅为15%-20%。