Center for Biostatistics, Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, United States of America.
Department of Radiation Oncology, The Ohio State University, Columbus, Ohio, United States of America.
PLoS One. 2019 Nov 21;14(11):e0225505. doi: 10.1371/journal.pone.0225505. eCollection 2019.
The NanoString nCounter System has been widely used in basic science and translational science research for the past decade. The System consists of two instruments: the PrepStation and the Digital Analyzer, and both have been continuously improved with evolving technologies. A great amount of research data have been generated at multiple research laboratories with the employment of different generations of the System. With the need of integrating multiple datasets, researchers are interested to know whether signals are comparable between different generations of the System. Toward this end, we designed a profiling study to compare performance of two generations of the NanoString nCounter System using a common set of biological samples. Using graphical tools and statistical models, we found that two different generations of NanoString nCounter System produced equivalent signals and signal deviations are in the range of random background noises for the medium-high expression levels.
过去十年中，NanoString nCounter 系统在基础科学和转化科学研究中得到了广泛应用。该系统由两个仪器组成：PrepStation 和 Digital Analyzer，并且随着技术的不断发展，这两个仪器都在不断改进。在多个研究实验室中，使用不同代的系统生成了大量的研究数据。随着整合多个数据集的需求，研究人员有兴趣了解不同代系统之间的信号是否具有可比性。为此，我们设计了一项分析研究，使用一组共同的生物样本比较两代 NanoString nCounter 系统的性能。使用图形工具和统计模型，我们发现两代不同的 NanoString nCounter 系统产生了等效的信号，并且信号偏差在中高表达水平的随机背景噪声范围内。
