Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic; Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Central European Institute of Technology, Masaryk University, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic.
Int J Biol Macromol. 2020 Jun 1;152:1113-1124. doi: 10.1016/j.ijbiomac.2019.10.200. Epub 2019 Nov 18.
Burkholderia pseudomallei and Chromobacterium violaceum are bacteria of tropical and subtropical soil and water that occasionally cause fatal infections in humans and animals. Microbial lectins mediate the adhesion of organisms to host cells, which is the first phase in the development of infection. Here we report the discovery of two novel lectins from the above-mentioned bacteria - BP39L and CV39L. The crystal structures revealed that the lectins possess a seven-bladed β-propeller fold. Functional studies conducted on a series of oligo- and polysaccharides confirmed the preference of BP39L for mannosylated saccharides and CV39L for rather more complex polysaccharides with a monosaccharide preference for β-l-fucose. The presented data indicate that the proteins belong to a currently unknown family of lectins.
类鼻疽伯克霍尔德菌和威克斯菌是热带和亚热带土壤和水中的细菌，偶尔会导致人类和动物的致命感染。微生物凝集素介导生物体与宿主细胞的黏附，这是感染发展的第一阶段。在这里，我们报道了从上述细菌中发现的两种新型凝集素——BP39L 和 CV39L。晶体结构揭示，凝集素具有七叶β-螺旋桨折叠。对一系列寡糖和多糖的功能研究证实，BP39L 优先结合甘露糖化的糖，而 CV39L 优先结合具有单糖β-l-岩藻糖偏好的更复杂的多糖。所提供的数据表明，这些蛋白属于一个目前未知的凝集素家族。
