College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou, 075000, China.
Plant Physiol Biochem. 2020 Jan;146:133-142. doi: 10.1016/j.plaphy.2019.11.015. Epub 2019 Nov 12.
Avena sativa L. is the most important cultivated oat species worldwide. Although photoperiod-insensitive oat varieties exist, the molecular mechanisms underlying their photoperiod sensitivity are poorly understood. This study investigated the effects of day length on the fioral transition of oats and the mechanisms underlying oat photoperiod insensitivity. Photoperiod-sensitive and photoperiod-insensitive varieties, including gp012, were used in shading experiments, and the developing leaves and main shoot apices (MSAs) of the HONGQI2 and gp012 varieties were used for sequencing. Leaves and MSAs were collected in 2016, and their transcriptomes were sequenced. The photoperiod-insensitive varieties headed under both short-day and long-day conditions, while the photoperiod-sensitive varieties headed only under long-day conditions. A total of 60673 transcript sequences were obtained, 7932 of which were differentially expressed; 3194 and 4738 transcripts were differentially expressed in the leaves and MSAs, respectively. A total of 25793 transcripts were classified into 123 pathways based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The carbon metabolism pathways were dominant, followed by ribosome and protein processing in the endoplasmic reticulum. In addition, 203 transcripts were classified into the circadian rhythm pathway. Compared with the expression of pseudo-response regulator protein 37 (PRR37) in photoperiod-sensitive varieties, that in photoperiod-insensitive varieties was upregulated. Among the differentially expressed transcripts (DETs), 8 MADS-box genes were identified. PRR37 is a key regulator of oat photoperiod insensitivity. The obtained transcriptome dataset may provide a reference for analyzing oat transcript expression, and the results should be used as a reference for oat breeding and production.
燕麦是世界上最重要的栽培燕麦种。虽然存在对光周期不敏感的燕麦品种，但对其光周期敏感性的分子机制知之甚少。本研究调查了长日条件对燕麦花转变的影响及其对燕麦光周期不敏感的机制。使用感光和非感光品种，包括 gp012，进行遮荫实验，并用 HONGQI2 和 gp012 品种的发育叶片和主茎顶端（MSAs）进行测序。2016 年采集叶片和 MSAs 进行转录组测序。非感光品种在短日和长日条件下都能抽穗，而感光品种仅在长日条件下能抽穗。共获得 60673 条转录序列，其中 7932 条差异表达；叶片和 MSAs 分别有 3194 和 4738 条差异表达的转录本。共 25793 条转录本根据京都基因与基因组百科全书（KEGG）数据库分为 123 条途径。碳代谢途径占主导地位，其次是核糖体和内质网中的蛋白质加工。此外，203 条转录本被归类为昼夜节律途径。与感光品种中拟反应调节蛋白 37（PRR37）的表达相比，非感光品种中 PRR37 的表达上调。在差异表达的转录本（DETs）中，鉴定出 8 个 MADS 框基因。PRR37 是燕麦光周期不敏感的关键调节因子。获得的转录组数据集可为分析燕麦转录本表达提供参考，结果可作为燕麦育种和生产的参考。
