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利用 RNA 测序对燕麦幼苗干旱胁迫扰动基因进行全球洞察。

Global insights to drought stress perturbed genes in oat (.) seedlings using RNA sequencing.

机构信息

Key Laboratory of Herbage & Endemic Crop Biotechnology, Ministry of Education, School of Life Science, Inner Mongolia University , , China.

Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences , Huhhot, Inner Mongolia, China.

出版信息

Plant Signal Behav. 2021 Feb 1;16(2):1845934. doi: 10.1080/15592324.2020.1845934. Epub 2020 Dec 23.

Abstract

Oat ( L.) is an important crop in northwestern China. Drought stress is the most significant factor affecting oat yield. In the present study, we explored the changes that occur in oats under drought stress conditions at a global genomic level. RNA sequencing was performed using 15-day-old oat seedlings. The differentially expressed transcripts were identified, and their related functions and pathways were investigated. In total, 1,065 unigenes were differentially expressed in oats under drought stress conditions. Of these, 386 unigenes were upregulated and 679 were downregulated. The perturbed transcripts were closely related to the biosynthesis of secondary metabolites, plant hormone signal transduction, and biosynthesis of antibiotics. DN50483_c0_g1_i3, which was annotated as acetyl-CoA carboxylase, was a significant node in the protein-protein interaction network. Biosynthesis of antibiotics and secondary metabolites may be involved in the drought stress response mechanisms of oats. The perturbed transcripts may provide targets for improving plant stress responses.

摘要

燕麦(L.)是中国西北地区的重要作物。干旱胁迫是影响燕麦产量的最重要因素。在本研究中,我们从全基因组水平上探讨了燕麦在干旱胁迫条件下的变化。使用 15 日龄的燕麦幼苗进行 RNA 测序。鉴定了差异表达的转录本,并研究了它们的相关功能和途径。在干旱胁迫条件下,燕麦中有 1065 个基因差异表达。其中,386 个基因上调,679 个基因下调。受干扰的转录本与次生代谢物的生物合成、植物激素信号转导和抗生素生物合成密切相关。被注释为乙酰辅酶 A 羧化酶的 DN50483_c0_g1_i3 是蛋白质-蛋白质相互作用网络中的一个重要节点。抗生素和次生代谢物的生物合成可能参与了燕麦对干旱胁迫的响应机制。受干扰的转录本可能为提高植物的应激反应提供了靶点。

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