Bio-products Research Chair, Department of Zoology, College of Science, King Saud University, P.O. Box 2455 Riyadh, Saudi Arabia.
J Microbiol Biotechnol. 2019 Jan 28;30(6):893-902. doi: 10.4014/jmb.1905.05027.
Propolis is a resinous substance that is collected by from plant sources and is used in traditional medicine. To study the phytochemical constituents and apoptotic potential of Jordanian propolis extract against different cancer cell lines, propolis was extracted using methanol, hexane, and ethyl acetate and was fractionated using chromatographic methods. Cytotoxicity was assessed using MTT and LDH assays. The apoptotic potential was investigated using florescence microscopy, multicaspase assay, Annexin-V and dead cell assay, and cell cycle assay. The phytochemical constituents were analyzed using GC-MS. The methanol extract of propolis exhibited cytotoxic potential against all cell lines tested. The IC values of the methanol extract were 47.4, 77.8, 91.2, and 145.0 μg/ml for HepG2, LoVo, MDAMB231, and MCF7 cell lines, respectively. The IC values of the F1 fraction were 31.6 (MDAMB231), 38.9 (HepG2), 36.7 (LoVo) and 75.5 (MCF7) μg/ml. On further purification using thin-layer chromatography, the IC values of the F1-3 fraction were found to be 84.31(HepG2), 79.2 (MCF7), 70.4 (LoVo), and 68.9 (MDAMB231) μg/ml, respectively. The anticancer potential of the F1 fraction was confirmed through the induction of apoptosis and cell cycle arrest at the G0/G1 phase. The GC-MS analysis of the F1 fraction revealed the presence of 3-methyl-4- isopropylphenol (29.44%) as a major constituent. These findings indicate the potential of propolis extract as a cancer therapy. However, further investigation is required to assess the acute and subacute toxicity of the most active fraction.
蜂胶是一种从植物源采集的树脂状物质，在传统医学中被使用。为了研究约旦蜂胶提取物对不同癌细胞系的植物化学成分和凋亡潜力，使用甲醇、己烷和乙酸乙酯提取蜂胶，并通过色谱方法进行分离。使用 MTT 和 LDH 测定法评估细胞毒性。通过荧光显微镜、多半胱氨酸酶测定法、Annexin-V 和死细胞测定法以及细胞周期测定法研究凋亡潜力。使用 GC-MS 分析植物化学成分。蜂胶甲醇提取物对所有测试的细胞系均显示出细胞毒性潜力。甲醇提取物对 HepG2、LoVo、MDAMB231 和 MCF7 细胞系的 IC 值分别为 47.4、77.8、91.2 和 145.0 μg/ml。F1 馏分的 IC 值分别为 31.6（MDAMB231）、38.9（HepG2）、36.7（LoVo）和 75.5（MCF7）μg/ml。在用薄层层析法进一步纯化后，发现 F1-3 馏分的 IC 值分别为 84.31（HepG2）、79.2（MCF7）、70.4（LoVo）和 68.9（MDAMB231）μg/ml。F1 馏分的抗癌潜力通过诱导细胞凋亡和细胞周期停滞在 G0/G1 期得到证实。F1 馏分的 GC-MS 分析显示存在 3-甲基-4-异丙基苯酚（29.44%）作为主要成分。这些发现表明蜂胶提取物作为癌症治疗的潜力。然而，需要进一步研究来评估最活跃馏分的急性和亚急性毒性。
