Bano Shaista, Siddiqui Bina Shaheen, Farooq Ahsana Dar, Begum Sabira, Siddiqui Faheema, Kashif Muhammad, Azhar Mudassar
a HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences , University of Karachi , Karachi , Pakistan.
b Department of Eastern medicine , Hamdard Al-Majeed College of Eastern Medicine Hamdard University , Karachi , Pakistan.
Nat Prod Res. 2017 Dec;31(24):2936-2940. doi: 10.1080/14786419.2017.1305380. Epub 2017 Apr 13.
Several Euphorbia species have been used in folklore as cancer remedies, however, scientific studies on the cytotoxicity (in vitro studies) of Euphorbia caducifolia are lacking. In present study, anticancer potential of E. caducifolia aerial parts ethanol extract and its fractions were evaluated against human lung (NCI-H460), breast (MCF-7), prostate (PC-3) and cervical (HeLa) cancer cell lines, using sulphorhodamine-B in vitro cytotoxicity (in vitro studies) assay. The ethanol extract demonstrated growth inhibitory effect against all aforementioned cancer cell lines with IC, 19-135 μg/mL and LC, ~220 μg/mL, and its petroleum ether fraction obtained on bioactivity guided fraction showed highest activity with IC, 28-70 μg/mL and LC, 71 μg/mL against NCI-H460 and MCF-7 cell lines. Its phytochemicals were analysed by gas chromatography-mass spectrometry (GC-MS). The present study provides scientific justification for its traditional use against cancer.
几种大戟属植物在民间被用作癌症治疗药物，然而，关于铁海棠（Euphorbia caducifolia）细胞毒性的科学研究（体外研究）尚属空白。在本研究中，采用体外细胞毒性（体外研究）测定法中的磺酰罗丹明 - B法，评估了铁海棠地上部分乙醇提取物及其馏分对人肺癌（NCI - H460）、乳腺癌（MCF - 7）、前列腺癌（PC - 3）和子宫颈癌（HeLa）细胞系的抗癌潜力。乙醇提取物对所有上述癌细胞系均表现出生长抑制作用，IC50为19 - 135μg/mL，LC50约为220μg/mL，并且在生物活性导向分级分离中获得的石油醚馏分表现出最高活性，对NCI - H460和MCF - 7细胞系的IC50为28 - 70μg/mL，LC5为71μg/mL。通过气相色谱 - 质谱联用（GC - MS）对其植物化学成分进行了分析。本研究为其传统的抗癌用途提供了科学依据。
