Yu R, Ni X Q, Ya Y, Fan G H
Department of Radiology, the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
Zhonghua Yi Xue Za Zhi. 2019 Nov 19;99(43):3428-3431. doi: 10.3760/cma.j.issn.0376-2491.2019.43.016.
To evaluate the value of Gd-BOPTA enhanced MRI in the staging of liver fibrosis. Fifty male SD rats (6-week-old, 180-220 g) were divided into the modeling group (42) and the control group (8). The model of liver fibrosis in the modeling group was established by carbon tetrachloride (animal license No. SYXK (Su) 2017-0043). From week 2 to week 10, rats in the modeling group (4) and control group (1) were randomly selected to scan 1 h(RER1), 2 h(RER2) and 3 h(RER3) after injection of Gd-BOPTA, respectively, to measure the relative enhancement rate (RER) of liver parenchyma. The shape of intrahepatic bile duct and the degree of enhancement at each time point were observed. Forty-two rats (34 rats in the modeling group and 8 rats in the control group) completed the experiment. RER1, RER2 and RER3 of the control group were 1.44±0.37, 1.22±0.37 and 0.84±0.28 respectively. RER1, RER2 and RER3 of the modeling group were respectively: S1 (6): 1.49±0.48, 1.29±0.39, 0.91±0.38;S2 (9): 1.48±0.44, 1.34±0.37, 1.04±0.40;S3 (11): 1.49±0.43, 1.37±0.39, 1.21±0.30; S4 (8): 1.49±0.44, 1.40±0.37, 1.24±0.40. There was no significant difference in RER1 and RER2 values between the control group and the liver fibrosis group (0.022, 0.999; 0.301, 0.875). There were significant differences between the control group and RER3 values of hepatic fibrosis stage S3 and S4 (2.249, 0.031; 2.274, 0.029), there was no significant difference between the remaining groups (all 0.05).In the control group, the intrahepatic bile duct was obviously strengthened within 1 hour after enhancement, and walked naturally. The intrahepatic bile duct was slightly enhanced 1h after the enhancement of S3-S4 stage of hepatic fibrosis, and the intrahepatic bile duct was significantly enhanced 2-3 hours later, with distorted alignment. Delayed 3 hours liver parenchymal RER and intrahepatic bile duct distortion delay enhancement after Gd-BOPTA enhancement contribute to the S3-S4 diagnosis of liver fibrosis.
评估钆贝葡胺增强磁共振成像（Gd-BOPTA enhanced MRI）在肝纤维化分期中的价值。选取50只雄性SD大鼠（6周龄，体重180 - 220 g），分为建模组（42只）和对照组（8只）。建模组采用四氯化碳建立肝纤维化模型（动物许可证号：SYXK(苏)2017 - 0043）。从第2周开始至第10周，分别随机选取建模组（4只）和对照组（1只）大鼠，在注射Gd-BOPTA后1小时（RER1）、2小时（RER2）和3小时（RER3）进行扫描，测量肝实质的相对增强率（RER）。观察肝内胆管形态及各时间点的强化程度。42只大鼠（建模组34只，对照组8只）完成实验。对照组的RER1、RER2和RER3分别为1.44±0.37、1.22±0.37和0.84±0.28。建模组的RER1、RER2和RER3分别为：S1期（6只）：1.49±0.48、1.29±0.39、0.91±0.38；S2期（9只）：1.48±0.44、1.34±0.37、1.04±0.40；S3期（11只）：1.49±0.43、1.37±0.39、1.21±0.30；S4期（8只）：1.49±0.44、1.40±0.37、1.24±0.40。对照组与肝纤维化组的RER1和RER2值无显著差异（0.022，0.999；0.301，0.875）。对照组与肝纤维化S3期和S4期的RER3值有显著差异（2.249，0.031；2.274，0.029），其余组间无显著差异（均P>0.05）。对照组增强后1小时内肝内胆管明显强化，走行自然。肝纤维化S3 - S4期增强后1小时肝内胆管轻度强化，2 - 3小时后肝内胆管显著强化，走行紊乱。Gd-BOPTA增强后延迟3小时肝实质RER及肝内胆管走行紊乱延迟强化有助于肝纤维化S3 - S4期的诊断。
