Zhang Xinying, Wang Denggang, Duan Yehong, Zheng Xueyun, Lin Ying, Liang Shuli
School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Biosci Biotechnol Biochem. 2020 Mar;84(3):463-470. doi: 10.1080/09168451.2019.1693250. Epub 2019 Nov 22.
Lycopene is a highly valued carotenoid with wide applications in various industries. The market demand for lycopene promotes research in metabolic engineering of heterologous hosts for lycopene. In this study, strain GS115 was genetically engineered to produce lycopene by integrating the heterologous lycopene biosynthesis genes from ATCC13032. The resulting strain, L, produced 0.115 mg/g cell dry weight (DCW) lycopene. Through optimization by promoter selection, improving the precursor supply and expanding the Geranylgeranyl diphosphate (GGPP) pool, ultimately, the lycopene yield of the final optimal strain was 6.146 mg/g DCW with shake flask fermentation and 9.319 mg/g DCW (0.714 g/L) in a 3 L fermenter. The lycopene yield in this study is the highest yield of lycopene in reported to date, which demonstrated the potential of in lycopene synthesis and as a candidate host organism for the synthesis of other high value-added terpenoids.
番茄红素是一种具有很高价值的类胡萝卜素，在各个行业都有广泛应用。市场对番茄红素的需求推动了利用异源宿主进行番茄红素代谢工程的研究。在本研究中，通过整合来自ATCC13032的异源番茄红素生物合成基因，对菌株GS115进行基因工程改造以生产番茄红素。所得菌株L产生了0.115 mg/g细胞干重（DCW）的番茄红素。通过启动子选择优化、改善前体供应和扩大香叶基香叶基二磷酸（GGPP）库，最终，最终优化菌株在摇瓶发酵中的番茄红素产量为6.146 mg/g DCW，在3 L发酵罐中的产量为9.319 mg/g DCW（0.714 g/L）。本研究中的番茄红素产量是迄今为止报道的最高产量，这证明了其在番茄红素合成中的潜力，以及作为合成其他高附加值萜类化合物的候选宿主生物的潜力。
