Sarwar Arslan, Lee Eun Yeol
Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin-si, Gyeonggi-do, 17104, Republic of Korea.
Synth Syst Biotechnol. 2023 Jun 13;8(3):396-415. doi: 10.1016/j.synbio.2023.06.001. eCollection 2023 Sep.
Methanol has recently gained significant attention as a potential carbon substrate for the production of fuels and chemicals, owing to its high degree of reduction, abundance, and low price. Native methylotrophic yeasts and bacteria have been investigated for the production of fuels and chemicals. Alternatively, synthetic methylotrophic strains are also being developed by reconstructing methanol utilization pathways in model microorganisms, such as Owing to the complex metabolic pathways, limited availability of genetic tools, and methanol/formaldehyde toxicity, the high-level production of target products for industrial applications are still under development to satisfy commercial feasibility. This article reviews the production of biofuels and chemicals by native and synthetic methylotrophic microorganisms. It also highlights the advantages and limitations of both types of methylotrophs and provides an overview of ways to improve their efficiency for the production of fuels and chemicals from methanol.
甲醇因其高还原度、丰富性和低价格，最近作为生产燃料和化学品的潜在碳底物受到了广泛关注。天然甲基营养型酵母和细菌已被研究用于生产燃料和化学品。此外，还通过在模式微生物（如……）中重建甲醇利用途径来开发合成甲基营养型菌株。由于代谢途径复杂、遗传工具可用性有限以及甲醇/甲醛毒性，用于工业应用的目标产品的高水平生产仍在开发中，以满足商业可行性。本文综述了天然和合成甲基营养型微生物生产生物燃料和化学品的情况。它还强调了这两种类型甲基营养菌的优点和局限性，并概述了提高它们从甲醇生产燃料和化学品效率的方法。
