Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
J Chromatogr A. 2020 Mar 15;1614:460704. doi: 10.1016/j.chroma.2019.460704. Epub 2019 Nov 13.
Profiling the spatial distributions and dynamic changes of metabolites in plant tissue is critical to elucidate the complex metabolic regulation during plant growth, development, and responses to abiotic or biotic stresses. In this study, we developed a high-coverage MALDI-MS imaging method to visualize the spatial locations of a wide spectrum of metabolites in Salvia miltiorrhiza Bge. 1,5-Diaminonaphthalene (DAN) and 1,1'-binaphthyl-2,2'-diamine (BNDM) were optimized as MALDI matrices in positive and negative ion modes respectively, due to their low background interference and high sensitivity. Moreover, a simple organic washing protocol using acetone was shown to significantly improve the sensitivity of MALDI-MSI. Using this method, we successfully imaged the spatial locations of amino acids, phenolic acids, fatty acids, oligosaccharides, cholines, polyamines, tanshinones, and phospholipids in Salvia miltiorrhiza Bge. In addition, the distributions of some metabolites in Salvia miltiorrhiza Bge root and stem were found to exhibit good spatial match with plant tissue structure. Thus, our method provides a spatially-resolved way to map the plant metabolic networks and to understand the physiological roles of several plant metabolites.
解析植物组织中代谢物的空间分布和动态变化对于阐明植物生长、发育以及对非生物或生物胁迫响应过程中的复杂代谢调控至关重要。在本研究中，我们开发了一种高覆盖的 MALDI-MS 成像方法，用于可视化丹参中广泛代谢物的空间位置。1,5-二氨基萘（DAN）和 1,1'-联萘-2,2'-二胺（BNDM）分别在正离子和负离子模式下被优化为 MALDI 基质，因为它们具有低背景干扰和高灵敏度。此外，使用丙酮的简单有机洗涤方案显示可显著提高 MALDI-MSI 的灵敏度。使用该方法，我们成功地对丹参中的氨基酸、酚酸、脂肪酸、低聚糖、胆碱、多胺、丹参酮和磷脂的空间位置进行了成像。此外，还发现丹参根和茎中一些代谢物的分布与植物组织结构具有良好的空间匹配性。因此，我们的方法为绘制植物代谢网络和理解几种植物代谢物的生理作用提供了一种空间分辨的方法。
